Sequences from confirmation amplicon


Confirmation sequences for line 212E06 and hit At1g52570 are shown.
Line ID 212E06
Locus At1g52570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19584442
Primers used for PCR KT69 - 8474, show primer details
   
Sequence Name 31-K100995-0022-212-E06C-8409
GenBank Accession LN500085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K100995-0022-212-E06C-8409
GGACTGAGCCTTTACATTGAATATATTGCTGAGTAAATGATGTTTATAACATTTACATAA
TTTATAACTTTTCAATACTAACCAAATGATTCAACATCAAAATTTTCGCAGTTGACGATG
AGTACATTATAATCGGATCAGCAAATATCAACCAGAGGTCAATGGATGGTGCAAGAGACT
CAGAGATAGCAATGGGAGGCTATCAACCATATCATCTCTCAACAAGACAACCAGCTCGAG
GTCAAATCCATGGATTCCGCATGTCTTTATGGTATGAACATTTAGGAATGCTCGATGAAA
CATTCCTCGATCCATCGAGCCAAGAATGTATCCNGAAAGTCAACCGTGTTGCTGATAAGT
ATTGGGATCTTTACTCAAGTGAGTCACTTGAACATGATCTTCCTGGTCATCTACTTCGCT
ACCCGATCGGTATTGC
   
Sequence Name 31-K100999-0022-212-E06C-KT69
GenBank Accession LN500086 [GenBank]
Sequencing primer gene specific primer, KT69: 5'-AGTGATGTTCCCTTCACTAGCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K100999-0022-212-E06C-KT69
GAAGATATGTTCAGTGACTCACTTGAGTAAAGATCCCAATACTTATCAGCAACACGGTTG
ACTTTCTGGATACATTCTTGGCTCGATGGATCGAGGAATGTTTCATCGAGCATTCCTAAA
TGTTCATACCATAAAGACATGCGGAATCCATGGATTTGACCTCGAGCTGGTTGTCTTGTT
GAGAGATGATATGGTTGATAGCCTCCCATTGCTATCTCTGAGTCTCTTGCACCATCCATT
GACCTCTGGTTGATATTTGCTGATCCGATTATAATGTACTCATCGTCAACTGCGAAAATT
TTGATGTTGAATCATTTGGTTAGTATTGAAAAGTTATAAATTATGTAAATGTTATAAACA
TCATTTACTCAGCAATATATTCAATTGTAAATGGCTTCAGGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11