Sequences from confirmation amplicon


Confirmation sequences for line 212G10 and hit At1g16710 are shown.
Line ID 212G10
Locus At1g16710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5720713
Primers used for PCR 05kt - 8474, show primer details
   
Sequence Name 46-K113916-0022-212-G10C-05kt
GenBank Accession LN505928 [GenBank]
Sequencing primer gene specific primer, 05kt: 5'-TTGCCTAGCTTCTTTATTCTGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K113916-0022-212-G10C-05kt
AACCCTTCTTTTCTGGAACAGCATTGCACACATCATAGTTCGGGCAAACTTTACAGCGCC
AGCCTAGACCACTTTCAATATCGAGGTGACATACGTTACATGTTGTCACAAAGGCAGGAG
CAGTAGGATTATGCAGATGATAAAGCACCATCATGGAAGAATGTTTTGCCCGCCTCAGTG
TATCATACTGATAATGGTTCCCTTGACATAGACTCAGGAATGCTTGCCTTGTATCAAAGA
ATTCACTTTCAAGTATCTCATCTCTGTCCTTAGTATCCGGACAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 18-K113915-0022-212-G10C-8409
GenBank Accession LN494389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113915-0022-212-G10C-8409
AAGTGAAATTTCTTTGAATACAAGGCAAGCATTTCTGAGTTCTATTGTTCAAGGGAAACC
ATTATCAGTATGATACACTGAGGCGGGCAAAACATTCTTCCATGATGGTGCTTTATCATC
TGCATAATCCTACTCGCTCCTGCCTTTGTGACAACATGTAACGTATGTCACCTCGATATT
GAAAGTGGTCTAGGCTGGCGCTGTGAAGTTTGCCCAGACTATGATGTGTGCAATGCTTGT
TACAAGAAAGAGGGTTGTATTAATCATCCTCATAAGTTAACAACTCATCCATCTCTAGCT
GATCAAAACGCTCAGAATAA

Last Updated on 06.12.2018 16:11