Sequences from confirmation amplicon


Confirmation sequences for line 213A12 and hit At3g54950 are shown.
Line ID 213A12
Locus At3g54950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20360085
Primers used for PCR JK51 - 8474, show primer details
   
Sequence Name 11-K100937-0022-213-A12C-8409
GenBank Accession LN491036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K100937-0022-213-A12C-8409
CATTTACAAACCTCCGAGAGCCTAAAATCATAGCCGTCCGTCTCAAGCGCATCGGCCCGT
GAGAACAGAAACGGGCCAGAGCTTTTGAGGTCGTAGCAAGGTATAAGCACCGGTTTAAGC
GTGTCCTTTAAGGTTAGCTCCGAGAAAGACTCTTTCATCACTTTCTTTAACTTAGCCGTC
CCGGAACAACAACCCGACCCGGTTCTCAAGACCCGTTTCAAGATTCCTGCTCCTCCGTAA
AGACCCTTGGCGTTTCTCGTTTAGAACTGCCACGTGTCATCT
   
Sequence Name 11-K100943-0022-213-A12C-JK51
GenBank Accession LN491037 [GenBank]
Sequencing primer gene specific primer, JK51: 5'-CTTTGGCTTATCTAGAACACGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K100943-0022-213-A12C-JK51
CCGAATCGCCGATACTTTGACGTAGCCGCCGGGTCCGGTATAGGTGGGATTTACCAGCCA
TGTTGTTCGGGTCAAGAGACGGTAACCGCCCCATCTTCAAAGCAGATGACACGTGGCAGT
TCTTAACGAGAAACGCCAAGGGTCTTTACGGAGGAGCAGGAATCTTGAAACGGGTCTTGA
GAACCGGGTCGGGTTGTTGTTCCGGGACGGCTAAGTTAAAGAAAGTGATGAAAGAGTCTT
TCTCGGAGCTAACCCTTAAGGACACGCTTAAACCGGTGCTTATACCTTGC

Last Updated on 06.12.2018 16:11