Sequences from confirmation amplicon


Confirmation sequences for line 213B04 and hit At3g27070 are shown.
Line ID 213B04
Locus At3g27070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9984006
Primers used for PCR DL30 - 8474, show primer details
   
Sequence Name 07-K018514-022-213-B04C-DL30
GenBank Accession LN489048 [GenBank]
Sequencing primer gene specific primer, DL30: 5'-TGACTCTTCCTTGGTTTGATTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K018514-022-213-B04C-DL30
TCAATGGCGGGAATTGAAAAATTTTCTAGCACTCTCCTTAACCTTGTATGTTTTTTTTGC
AGAATTTGATGAGATGGGGAGAAGCTTTACTAGAGTTATCTCAGTTTCAAAACGTTATAG
ATTCACTGAAAATGATTCAAGGCGTATTTTTTTTTTTTTTTTGGG
   
Sequence Name 08-K018514-022-213-B04C-8409
GenBank Accession LN489516 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K018514-022-213-B04C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATGCATTCCCCAAGCACCAAACCGC
ATCATGTTTCATTGGGTCAATCAATATTGCATCTTCGAGTTTTGAGATGGCATCTACGCA
AAGAAAATAAAATTGTAACTAAAGCAGTTTTTCATCACAATTAATTTACATAAGCATGAA
GCATCATCATACCTACAAGAGCTTGAAACATTAATAAAACAAGTTCAAGTTTTTAAAGAA
GACATAAGTGATATTACAATACTAAAAACATTTGATTCCTATAATGGTTAGGGAATCCAA
AATGTGCAATATTCAATTATGTTAAGTTATTAACACATAAAGCAAACATTTACCACCAAA
AAAAAAAAAAAAAATCCCCCTGGAATCTTTTTCGGGGAATCTAAAACGTTTGAAAACGGA
AAAACCTCTAGAAAAGTTTCCCCCCTTCTCATAAATTTCGGCAAAAAAAACTTCCAGGGT
TAGGGAAAGGGCTAAAAATTTTTTCATTCCCCCCCTTGGTTTCCAAAACCAAAATTCAAC
CCGGGGGG

Last Updated on 06.12.2018 16:11