Sequences from confirmation amplicon


Confirmation sequences for line 213B06 and hit At1g15730 are shown.
Line ID 213B06
Locus At1g15730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5407720
Primers used for PCR 10dr - 8474, show primer details
   
Sequence Name 22-K110172-0022-213-B06C-10dr
GenBank Accession LN496157 [GenBank]
Sequencing primer gene specific primer, 10dr: 5'-CACCGTTGAAGAAAGGAAGATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110172-0022-213-B06C-10dr
ATGAATTCTGGACTTCTGAAGAGTGGCAGCTTTGAGGGAAGGAGTTAAAGAATATGGAAA
ATCATGGAAAGATATCAAGAATTCATACCCTGTTGTTTTCGCTGATAGGAGCGAGGTGGA
TTTGAAGGATAAGTGGAGGAATTTACTTGGTCGTCAGTAGTAGGAATCTTACCTTCTAAC
TTATGGGCATTTTGGTTTTGGGATCTTGTGTAGTTTTGCCGCTTGTGTTAGAAAGGGACT
CACTTTTTGTACGGTGAAGGCTTTAATATGTTCTTGATTCAGGCTCTTGTAGTTAGTGTC
AAGCTTTAGACGATATTTCTTGTTCAAAAATCTATTGACCAGTTGATTACAAAATGGAAT
GATTTTAAATATTTGGTCCTATAATCAAATCAAGCAAGCTCTGAAACCCATCTCTAACTC
CTCCCGGTTCAAATTCTTGCCGATGAAAACAATCTTGTTGGTTCTTGTTTCATCTTTTCT
CCATAACCGGTCAGGTGATCCTTCAAATATTTCATGAACTCCCTGTTCCAACCATTATAA
ACCACAAACGTCAGAGATAATTTACATCCCTGTTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGC
   
Sequence Name 06-K110171-0022-213-B06C-8409
GenBank Accession LN488860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110171-0022-213-B06C-8409
TTACAATTGAACAGGGATGTAAATTATCTCTGACGTTTGTGGTTTATAATGGTTGGAACA
GGGAGTTCATGAAATATTTGAAGGATCACCTGACCGGTTATGGAGAAAAGATGAAACAAG
AACCAACAAGATTGTTTTCATCGGCAAGAATTTGAACCGGGAGGAGTTAGAGATGGGTTT
CAGAGCTTGCTTGATTTGATTATAGGACCAAATATTTAAAATCATTCCATTTTGTAATCA
ACTGGTCAATAGATTTTTGAACAAGAAATATCGTCTAAAGCTTGACACTAACTACAAGAG
CCTGAATCAAGAACATATTAAAGCCTTCACCGTACAAAAAGTGAGTCCCTTTCTAACACA
AGCGGCAAAACTACACAAGATCCCAAAACCAAAATGCCCATAAGTTAGAAGGTAAGATTC
CTACTACTGACGACCAAGTAAATTCCTCCACTTATCCTTCAAATCCACCTCGCTCCTATC
AGCGAAAACAACAGGGTATGAATTCTTGATATCTTTCCATGATTTTCCATATTCTTTAAC
TCCTTCCCTCAAAGCTGCCACTTCTTCAGAAGTCCAGAATTTCATTGGCCTTCTGGGACG
CGGTCTTTCTAATCTTCCTTTCTTCCACGGTG

Last Updated on 06.12.2018 16:11