Sequences from confirmation amplicon


Confirmation sequences for line 213B12 and hit At1g76110 are shown.
Line ID 213B12
Locus At1g76110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28557157
Primers used for PCR 13aq - 8474, show primer details
   
Sequence Name 13-K106755-0022-213-B12C-8409
GenBank Accession LN492057 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106755-0022-213-B12C-8409
GCCATTTACAATTGCATTTCACGAGGAGGGGAGGTTACGAAAAGGTCAACACTTTTTGTT
TTCTTTTTCAATACAGATATTTTTTTACTTTATGGTAATTTATTATCGTTTCTTATATGT
TGTGTTGTTTTGAAAATGCATGTTGAATTGATAGGTAGTCGTAGAGAAAAAATGGAGGGA
AGTTGGAGGAGTGTTTAGATTCTCTGCGACAACAACAAGTGCTTCGTTTGTCTTAAGGAA
ACATTATCTCAATCTTCTCTTCCATTACGAACAAGTTCATCTCTTCACTGCTCGTGGTCC
TCTTCTTCATCCCATAGGTTTGTTTTTTTTTCTCAAACCTTCTTCAATTGATTACTTTTA
AATATATATAGTATACATTTCATTATATTCTTCAATTATCATAGATTTGTTTCTGTTGAG
AATCTTTAATTCAACCGTTTGATAAATGCTAATCAATGAATCATAAGATGGTTCCCATAT
TGTCTTTAATGCCCTAGGACTAGAAAGTTCAATTCGTTGTTACTCATTAACGATAGTCTG
AAAGGCTTGAATCTATAATAAAATAATAAGAATAACTTTTCAATTATGTGAAGTCATGGG
TTCAATCGTGGGAC
   
Sequence Name 29-K106756-0022-213-B12C-13aq
GenBank Accession LN499307 [GenBank]
Sequencing primer gene specific primer, 13aq: 5'-TTTGTAGTAAGAATGTGTCCCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106756-0022-213-B12C-13aq
ATTGAAAGTATTCTTATTATTTTATTATAGATTCAAGCCTTTCAGACTATCGTTAATGAG
TAACAACGAATTGAACTTTCTAGTCCTAGGGCATTAAAGACAATATGGGAACCATCTTAT
GATTCATTGATTAGCATTTATCAAACGGTTGAATTAAAGATTCTCAACAGAAACAAATCT
ATGATAATTGAAGAATATAATGAAATGTATACTATATATATTTAAAAGTAATCAATTGAA
GAAGGTTTGAGAAAAAAAAACAAACCTATGGGATGAAGAAGAGGACCACGAGCAGTGAAG
AGATGAACTTGTTCGTAATGGAAGAGAAGATTGAGATAATGTTTCCTTAAGACAAACGAA
GCACTTGTTGTTGTCGCAGAGAATCTAAACACTCCTCCAACTTCCCTCCATTTTTTCTCT
ACGACTACCTATCAATTCAACATGCATTTTCAAAACAACACAACATATAAGAAACGATAA
TAAATTACCATAAAGTAAAAAAATATCTGTATTGAAAAAGAAAACAAAAAGTGTTGACCT
TTTCGTAACCTCCCCTCCTCGTGAAATGCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTC
Primers used for PCR 01bd - 3144, show primer details
   
Sequence Name 03-K107108-0022-213-B12C-3144
GenBank Accession LN487507 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K107108-0022-213-B12C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAAAATATCT
GTATTGCAAATCTAGTTCTTTTCCTCCAATCACAGGAATCCTTGTTTATATCACACAAAA
ATAATAAAATAAGATAGATGAAATGACGAAAACTAAATTTATTAAGTAAGAAGAAGAGAA
AGAATAATGGTTACATGAACTTAGTGGACATAATTGAGTGAAACCGCCTGAGAGTATCCC
AGAAGACAGAAGAATCCTTGACGACNACTTCATGTAAAGCCAATGGTTCTGGATACTCTT
TAATCTTCGCCGGCGAAGTCGCCATCATTTCTACCGTCGCTTGAGACGGTGTCGATTCGT
TGTCCGATGACATCGCTATAGAGAGACTTTTAGTAGAGACAGATATCTTTTTTTTGTTGG
GGAGTTTTTTTTTTTTGGCCGGGCCCCCCTTTTTGGGGTTTGGTTTTAGGGAAAAAAAAA
AAAAATACCTTTCCAAAAAAAAATTTGGGTTTAATTCAAAAAAATTTAGAATTAAAAATT
TAATTTTTTTTTTTTTCCACCGAATAAAAAAA
   
Sequence Name 19-K107113-0022-213-B12C-01bd
GenBank Accession LN494781 [GenBank]
Sequencing primer gene specific primer, 01bd: 5'-CCGTTACTTTTAAATCCGTAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K107113-0022-213-B12C-01bd
CTCTCTGATTAATCACAAATCTTATCTTGAATCGTTATCTTTCTCTTCTTTCCTTAAACC
AAAACCCAAAAAAGAGAGACCAGACAAAAAAAAAAAACCCCCCCACCAAAAAAAAAATTT
TGGTCTCTCCAAAAGTTCTCTTTTTGGGGTTTTCTTGGAAAAAGAATTCAACCCGTTTTA
AGGGGAGGGTAAAATGGGGGGCACTTTCCCGGGCAAAATTAAAGGATTTCCCAAACCCTT
GGGTTTTCCTGGAGTTTGCCTCCAGGGTTTTTTTGGTTTTGGGGGATCCCCTGGGGGGGT
TTCCCCCATTTTGGCCCCCAAGTTTCGGGAACCCTTTTTTTTTTCTTTTTTTTTACTTAA
AAAATTTTGTTTTCCGCCTTTCCCCCTTTTTATTTTTTTTTTTTTGGGGGGAAAAAAACA
GGGTTCCCGGGGTTGGGGGAAAAAAACTTAATTTGGCATTCCAATTTTTTTTCAACCCTG
GTTGTTTAAACCCAAGGGGGGGAACCAACATTTGGTCCCCGGGGTCCCTGGCCCTCCCCA
ATGAAATGGACTTCCTTTTTTTAGGGAAGGGTTTTGGCAAGGAAAGGGGGATTTGGGCTT
CTCCCTTACCTTCGGGGGAAATTT

Last Updated on 06.12.2018 16:11