Sequences from confirmation amplicon


Confirmation sequences for line 213C08 and hit At3g61040 are shown.
Line ID 213C08
Locus At3g61040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22594554
Primers used for PCR MS57 - 8474, show primer details
   
Sequence Name 42-K017191-022-213-C08C-MS57
GenBank Accession LN504284 [GenBank]
Sequencing primer gene specific primer, MS57: 5'-AGCTAGAAAAGAAGCGAGGCTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K017191-022-213-C08C-MS57
GATGCACAAACTGTTTAGGGTTTTTCAAGGATTCATCGATACAAAGAGATCATCAACTTC
GAGAAATAACAACGATATGTTAGATTCTCTTTTGGATATTGCTCATAAGAAGGAGTCAGA
ACTCGATGACAATAATATCAAACATCTGCTTCTCGTAAGTCTTAAATAAGCCAAAACAAA
TTGGCTTCTATACATGTTCTTAGCTAAATCCATGTAATTGTGTATTAGGACTTGTTTCTG
GCGGGAGTAGACACGAGTTCGAGTGCGGTGGAATGGGCTATGGCGGAGTTACTTCGAAAC
CCTAAGATGATAGTGAAAGTTCAAGAAGAGATAAGACAAGTGATAGGGCTAAAGGGCACC
GTTCAAGATCTTGATATCGTTAAACTACCTTATTTACAAGCAGTTGTGAAAGAGAGTCTT
CGTTTGCATCCGCCTGCTCCTTTCCTCGTCCCTCGAAAATCAGAGTCTGATGATGTCCAA
ATTTTCGAGTTCCTCATTCCTAAGAATACTCATTGCTGATCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 46-K017191-022-213-C08C-8409
GenBank Accession LN505710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K017191-022-213-C08C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGATCAGCAATGAGTATTCTTAGGA
ATGAGGAACTCGAAAATTTGGACATCATCAGACTCTGATTTTCGAGGGACGAGGAAAGGA
GCAGGCGGATGCAAACGAAGACTCTCTTTCACAACTGCTTGTAAATAAGGTAGTTTAACG
ATATCAAGATCTTGAACGGTGCCCTTTAGCCCTATCACTTGTCTTATCTCTTCTTGAACT
TTCACTATCATCTTAGGGTTTCGAAGTAACTCCGCCATAGCCCATTCCACCGCACTCGAA
CTCGTGTCTACTCCCGCCAGAAACAAGTCCTAATACACAATTACATGGATTTAGCTAAGA
ACATGTATAGAAGCCAATTTGTTTTGGCTTATTTAAGACTTACGAGAAGCAGATGTTTGA
TATTATTGTCATCGAGTTCTGACTCCTTCTTATGAGCAATATCCAAAAGAGAATCTAACA
TATCGTTGTTATTTCTCGAAGTTGATGATCTCTTTGTATCGATGAATCCTTGAAAAACCC
TAAACAGTTTGTGCATCAATAGCCTCGCTTC

Last Updated on 06.12.2018 16:11