Sequences from confirmation amplicon


Confirmation sequences for line 213D12 and hit At4g21600 are shown.
Line ID 213D12
Locus At4g21600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11485902
Primers used for PCR 11fe - 8474, show primer details
   
Sequence Name 51-K111227-0022-213-D12C-11fe
GenBank Accession LN507538 [GenBank]
Sequencing primer gene specific primer, 11fe: 5'-GGATCATGCTTTAGACTGAAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111227-0022-213-D12C-11fe
CATTTCCAATCCTCAGGATTCTTTGAGGATGATACTATTGCTGCAGTGAAGAAACTTCTG
CCTGAATCAGTTGATGGTGGTGGACTAGCAGATTTTTGCTCATGGCCTGATGAAATTAAA
AAACTCTCGCAATGGCAATGGACTAGCACTTTGCACTATGTTAACACACCTGAATACAGG
TGCAACTACGAGTATTGCCGTGGGTTATCTTTTCCTCCAGCTTCATACATGTTTCTGTTC
TTTTTTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 35-K111226-0022-213-D12C-8409
GenBank Accession LN501873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111226-0022-213-D12C-8409
TCATTGAATATATCCTGATAAAAAGACAGAAACATGTATGAAGCTGGAGGAAAAGATAAC
CCACGGCAATACTCGTAGTTGCACCTGTATTCAGGTGTGTTAACATAGTGCAAAGTGCTA
GTCCATTGCCATTGCGAGAGTTTTTTAATTTCATCAGGCCATGAGCAAAAATCTGCTAGT
CCACCACCATCAACTGATTCAGGCAGAAGTTTCTTCACTGCAGCAATAGTATCATCCTCA
AAGAATCCCTGAGGATTTGGAAATTGAAATCATCTTATCAACAAAGCTAATACCTTTTCA
GTCTAAANCATGATC

Last Updated on 06.12.2018 16:11