Sequences from confirmation amplicon


Confirmation sequences for line 213F07 and hit At5g05290 are shown.
Line ID 213F07
Locus At5g05290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1569598
Primers used for PCR KS01 - 8474, show primer details
   
Sequence Name 53-K020131-022-213-F07C-KS01
GenBank Accession LN508006 [GenBank]
Sequencing primer gene specific primer, KS01: 5'-GAGAATCAACGACGTCAAGACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K020131-022-213-F07C-KS01
CAAATTTGTCATTCTCATGACTAAAAGTCACGTGTGATGACTTCTTTATTTAATTATTAT
TCTTTTTCTTTAAAAGAAATTTAGTGTTGTTGTATATATATATATCAAATACTATAGTTG
TGTTACTACTGAAATTAAAATGTTTGAATTGCTGGCTTTTGGTTCTTCGTACTAGAATTT
TCAAATAGAGATACCTTTTGTACTAAGCGAGGATATGCGAAGAATACAAAATGATCTCCA
AACAAAATTAATTGTTTCATTGTATTTCTTTCTTATGACTATTATATATCGAAGAGTGGT
TATGATCAATGAAAAGCAGAGTTTTATCTTAAACACAATCATTGACTTTTCTTGGATATA
ACGTCTATTAGTTTCACTTGATCATAAATCTAAAATTGTCCGCCTTCAAAAGTCTGACCG
AACTGCCAATCATGAGGCACAACATCGTAGCTCACAACAGTCCGACCATCACTATCAGTG
ACTTGGAAAGAAAGGCTTTGACCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATG
   
Sequence Name 54-K020131-022-213-F07C-8409
GenBank Accession LN508338 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K020131-022-213-F07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAGGTCAAAGCCTTTCTTTCCAAGTCACTGATA
GTGATGGTCGGACTGTTGTGAGCTACGATGTTGTGCCTCATGATTGGCAGTTCGGTCAGA
CTTTTGAAGGCGGACAATTTTAGATTTATGATCAAGTGAAACTAATAGACGTTATATCCA
AGAAAAGTCAATGATTGTGTTTAAGATAAAACTCTGCTTTTCATTGATCATAACCACTCT
TCGATATATAATAGTCATAAGAAAGAAATACAATGAAACAATTAATTTTGTTTGGAGATC
ATTTTGTATTCTTCGCATATCCTCGCTTAGTACAAAAGGTATCTCTATTTGAAAATTCTA
GTACGAAGAACCAAAAGCCAGCAATTCAAACATTTTAATTTCAGTAGTAACACAACTATA
GTATTTGATATATATATATACAACAACACTAAATTTCTTTTAAAGAAAAAGAATAATAAT
TAAATAAAGAAGTCATCACACGTGACTTTTAGTCATGAGAATGACAAATTTGCTAAGTCT
TGACGT

Last Updated on 06.12.2018 16:11