Sequences from confirmation amplicon


Confirmation sequences for line 213H10 and hit At1g12940 are shown.
Line ID 213H10
Locus At1g12940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4417447
Primers used for PCR GL02 - 8474, show primer details
   
Sequence Name 32-K016600-022-213-H10C-8409
GenBank Accession LN500367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K016600-022-213-H10C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGGAGATTATT
GGGCGATGCATAAATCTGGAGAGAGGGAGAAAGATGATGTGGGGAAAGTGATATCTAATG
GAATCAAAAACTATAGGGGATGGATAACAGCATTAGCATATGGCTATTGTTTTGGAGTAG
AGCTTACCATTGACAACATCATCGCAGAATATTTCTTCGATAGATTCCATTTAAAGCTCC
AGACAGCAGGGATTATAGCAGCGAGTTTTGGACTAGCCAATTTTTTCGCTAGACCTGGAG
GAGGAATTTTCTCTGATTTTATGTCGAGACGGTTTGGGATGAGAGGAAGGTTGTGGGCTT
GGTGGATTGTGCAAACATCAGGAGGTGTATTATGCGCATGTCTTGGCCAGATTTCTTCCT
TGACAGTGTCTATAATTGTTATGCTTGTCTTCTCTGTATTCGTCCAAGCCGCTTGTGGAC
TTACCTTTGGCGTTGTTCCCTTTATTTCTAGAAGGTAACATACATTTTGGTATTGAGTT
   
Sequence Name 34-K016600-022-213-H10C-GL02
GenBank Accession LN501211 [GenBank]
Sequencing primer gene specific primer, GL02: 5'-CAATCAAGCAACTCAATACCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K016600-022-213-H10C-gl02
CTTACCTTCTAGAAATAAAGGGAACAACGCCAAAGGTAAGTCCACAAGCGGCTTGGACGA
ATACAGAGAAGACAAGCATAACAATTATAGACACTGTCAAGGAAGAAATCTGGCCAAGAC
ATGCGCATAATACACCTCCTGATGTTTGCACAATCCACCAAGCCCACAACCTTCCTCTCA
TCCCAAACCGTCTCGACATAAAATCAGAGAAAATTCCTCCTCCAGGTCTAGCGAAAAAAT
TGGCTAGTCCAAAACTCGCTGCTATAATCCCTGCTGTCTGGAGCTTTAAATGGAATCTAT
CGAAGAAATATTCTGCGATGATGTTGTCAATGGTAAGCTCTACTCCAAAACAATAGCCAT
ATGCTAATGCTGTTATCCATCCCCTATAGTTTTTGATTCCATTAGATATCACTTTCCCCA
CATCATCTTTCTCCCTCTCTCCAGATTTATGCATCGCCCAATAATCTCCATCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTAC

Last Updated on 06.12.2018 16:11