Sequences from confirmation amplicon


Confirmation sequences for line 214B03 and hit At1g20380 are shown.
Line ID 214B03
Locus At1g20380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7062183
Primers used for PCR 28by - 8474, show primer details
   
Sequence Name 23-K108223-0022-214-B03C-28by
GenBank Accession LN496587 [GenBank]
Sequencing primer gene specific primer, 28by: 5'-AGCTGGATAGCGACTGTAGAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K108223-0022-214-B03C-28by
ACTTACCTGATTTCGAACAAGTTTCATGTCAATGTCAATAATGAAACTTCAAACTAACGA
GTAATTCAACTTGAAGACGCCTTACCCAAGATAAAGAATCAGGTTCCACTTTCTTATCTT
CAATCTTCATCACTTTAATAGTAACCCAATCACTACCACTAGAGCTAAGGCCATAAGCCA
AATACTTAGCATCCTCACTAATAGATAAAGTGTTTAAAGAAACTGTTCCATCATCACTAA
GAGTATTAGGATCAAGCAAGATCTCTGCTTCAGACTCCAAATCATCCTTCAAATGAAAAA
AAAAAAAAATTCAAATCAAAACCCCCTTTAAAAAAAATTATCCCCGGCTTCAAATTCCCG
GAACATGAAGCACCCTCTGAGCTTGAAAACCTGATTTATGGAAGTAAAAGTACCTTTCCC
CCCCCTAAAGGGGCGTATCGAACCGAGAATAATCAATAAACTTTGGGAATTTGTCAGGAA
GCTTTTCCTTCGTTTCCCAACTTTGGAGCACTGAATCTAACAATGGCACTTGTTTCTCTA
TTTCCAATTGAAAAGGGCTTCATGTCCGGGAAATCTACTGGGATCACCATTGAGTATAAG
GGTCAATATGGAAAAAAAAAAAAAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAA
TTTC
   
Sequence Name 07-K108222-0022-214-B03C-8409
GenBank Accession LN489306 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K108222-0022-214-B03C-8409
ATTTACATTGAATAAGAGAAACAGTGCAATTGTCAGATTCAGTGCTCAATAGTTGCGAAA
CGAAGGAAAAGCTTCATGACAAATTCACAAAGTTTATTGATTATCCTCGTTTCGATACGC
CCTTTAAGCGAGGGAACAGCTACTTTTACTTCCATAACTCAGGTCTTCAAGCTCAGAGTG
TGCTTCATGTTCAGGTAATTTGAAGCAGAGTATAATCTTCTTTAAGTGGGTTTTTGATTT
GAATTTTTTTTTTTTTTCTTTTAAAGGATGATTTGGAGTCTAAAGCAAAAATTTGGCTGG
ATCCTAAAACTCTAAGGGATGAGGGAACATTTTCTTTAAACCCTTTTTCTTTAAGGGAGG
ATGCTAAGTATTGGGCTTAGGGCCTTACCTCTAGGGGTAGGGATGGGGTTACTTTTAAAG
GGATAAAAATTGAAAATAAAAAAGGGAAACCTGATTCTTTATCTGGGGAAAGGCGTCTTC
AAGTTAAATTACCCGTTAGTTGGAAGTTTCTTTATGGACTTTGACATGAAACTTGTTCTA
AAATCGGGTAAATTTTAGGGGGATCCCAGGGACACTTGAGGGGAAAGGTTTTTTCTACGG
TCCCTATCCA

Last Updated on 06.12.2018 16:11