Sequences from confirmation amplicon


Confirmation sequences for line 214G03 and hit At3g07070 are shown.
Line ID 214G03
Locus At3g07070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2239458
Primers used for PCR BM88 - 8474, show primer details
   
Sequence Name 08-K104415-0022-214-G03C-BM88
GenBank Accession LN489716 [GenBank]
Sequencing primer gene specific primer, BM88: 5'-TTGCCTCTTCTTGTAAACACATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104415-0022-214-G03C-BM88
GGGACCTCCCTCCGACTCGGATCCGCTAACTCTGGGAATCTACTCGGTTCTTTGAACACC
GGTTGAGCCTAAACCAAAGTACAAAAGTTTAGATCTTTTAAACAATTGAAGCCAAAAGGT
TTTCATGTAAGCTGAAAAAGAGAAACTCACCCAAGTTACTAGATTCTGTTCATCTTTGGG
TCTGGTAGTATCAATAACTCTTCGTCCAGTTATCAACTCCAACAACACAACTCCGAAGCT
ATAAACATCGGATTTTGTAGTTAACTGACCGGTTCTCTGATACTCTGGAGCACAATATCC
GTAAGTTCCCATAACTCTGGAAGACTACATGTCCCCGGGTTACAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 24-K104416-0022-214-G03C-8409
GenBank Accession LN496977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104416-0022-214-G03C-8409
CCATTTACAATTGTAACCCGGGGACATGTAGTCTTCCAGAGTTATGGGAACTTACGGATA
TTGTGCTCCAGAGTATCAGAGAACCGGTCAGTTAACTACAAAATCCGATGTTTATAGCTT
CGGAGTTGTGTTGTTGGAGTTGATAACTGGACGAAGAGTTATTGATACTACCAGACCCAA
AGATGAACAGAATCTAGTAACTTGGGTGAGTTTCTCTTTTTCAGCTTACATGAAAACCTT
TTGGCTTCAATTGTTTAAAAGATCTAAACTTTTGTACTTTGGTTTAGGCTCAACCGGTGT
TCAAAGAACCGAGTAGATTCCCAGAGTTAGCGGATCCGAGTCTGGAGGGAGTGTTCCCGG
AGAAAGCTCTGAATCAAGCGGTGGCAGTGGCAGCAATGTGTTTACAAGAAGAGGCA

Last Updated on 06.12.2018 16:11