Sequences from confirmation amplicon


Confirmation sequences for line 214G06 and hit At2g29490 are shown.
Line ID 214G06
Locus At2g29490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12631617
Primers used for PCR NK14 - 8474, show primer details
   
Sequence Name 87-K115121-0022-214-G06C-NK14
GenBank Accession LN514792 [GenBank]
Sequencing primer gene specific primer, NK14: 5'-AAGAACAGTCCAATTCTTCCTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K115121-0022-214-G06C-NK14
ATTTGGGCCAGTTCATCGATGACCAGGTATACTAATATTGATTTGCAGATTAATTAGTTG
ATTCTATGCAGAACAATATTATATTATTCGCTTGTTTATATATTGTTCTTTACTTGCAGA
TCCTAACACTAGGGTTTAGGTCCCTTGTAAAAGCAGAGAAAGGAAGAGAGGTTGCTATTG
AAGAGACTCGGGAACTGCTTATGTTTCTTGAGAAAGAAGTCACTGGAAAAGATTTCTTCG
GTGGCAAGACAATCGGATTCTTGGACATGATCGCAGGAAGTATGATCCCATTTTGTCTAG
CTAGACTTTGGAAAGGTATTGGAATTGATATGATTCCAGAGGAGAAGTTTCCGGAATTAA
ATAGATGGATCAAGAATTTGGAAGAAGTTGAGGCAGTGAGGGGATGTATTCCTCCAAGAG
AGAAACAAATTGAGCGCATGACCAAAATTAAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
   
Sequence Name 39-K115120-0022-214-G06C-8409
GenBank Accession LN503460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115120-0022-214-G06C-8409
TTACATTTAATTTTGGTCATGCGCTCAATTTGTTTCTCTCTTGGAGGAATACATCCCCTC
ACTGCCTCAACTTCTTCCAAATTCTTGATCCATCTATTTAATTCCGGAAACTTCTCCTCT
GGAATCATATCAATTCCAATACCTTTCCAAAGTCTAGCTAGACAAAATGGGATCATACTT
CCTGCGATCATGTCCAAGAATCCGATTGTCTTGCCACCGAAGAAATCTTTTCCAGTGACT
TCTTTCTCAAGAAACATAAGCAGTTCCCGAGTCTCTTCAATAGCAACCTCTCTTCCTTTC
TCTGCTTTTACAAGGGACCTAAACCCTAGTGTTAGGATCTGCAAGTAAAGAACAATATAT
AAACAAGCGAATAATATAATATTGTTCTGCATAGAATCAACTAATTAATCTGCAAATCAA
TATTAGTATACCTGGTCATCGATGAACTTGGCCCAAAATCGAGCCATGGCCTTCTCGTAA
GGATCTTGAGGAAGAATTGGACTGTTCT

Last Updated on 06.12.2018 16:11