Sequences from confirmation amplicon


Confirmation sequences for line 215D11 and hit At1g29030 are shown.
Line ID 215D11
Locus At1g29030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10132027
Primers used for PCR CS28 - 8474, show primer details
   
Sequence Name 64-K031320-022-215-D11C-8409
GenBank Accession LN511120 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K031320-022-215-D11C-8409
TTACAATTGAATATATCCTGGAGTTCAATTGATTAAATCGAAAAGGTTAGAACATATCTT
TGGCTGCATGGAGTTTTTCTTTGTACCTATTTATCTGGATTTGTTTTTAGCCCTTTTATG
ATGTTAACAGAGCCTAGGGGATGTAACTGGAGAAGAGTTCAATATGTTTATGGATTTCCT
GAAAAGTTTGAGTATATTTGGAGGGAAAGCTCCTCAAGAAAGAATGCAAGAACTTGTAGA
AATTATTGAAGGACAGGCTGATTTGGATTCAGAGTTTAATGTGAGTTTCTCGGTCACATT
CTTTTGGGCATGAACTTAATTTCTTACTTTAATACAATGGCAGTAAAATGATTTCATTCC
TCGTAGATGTTTATTGATTTTTAAATCACCCAAGTGGTCGTCGTTATCTCGGAATGAAGT
ATGCTAGTTTTTTGGGTGTTTGTGAGATTAAAAGGATACTTCTCCTGGCACAGGCTTTCA
CCCTCTCAACAAAAAGCTTAT
   
Sequence Name 65-K031320-022-215-D11C-CS28
GenBank Accession LN511319 [GenBank]
Sequencing primer gene specific primer, CS28: 5'-ATAAGCTTTTTGTTGAGAGGGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K031320-022-215-D11C-CS28
TTTTATCTCCAACACCCAAAAACTAGCATACTTCATTCCGAGATAACGACGACCACTTGG
GTGATTTAAAAATCAATAAACATCTACGAGGAATGAAATCATTTTACTGCCATTGTATTA
AAGTAAGAAATTAAGTTCATGCCCAAAAGAATGTGACCGAGAAACTCACATTAAACTCTG
AATCCAAATCAGCCTGTCCTTCAATAATTTCTACAAGTTCTTGCATTCTTTCTTGAGGAG
CTTTCCCTCCAAATATACTCAAACTTTTCAGGAAATCCATAAACATATTGAACTCTTCTC
CAGTTACATCCCCTAGGCTCTGTTAACATCATAAAAGGGCTAAAAACAAATCCAGATAAA
TAGGTACAAAGAAAAACTCCATGCAGCCAAAGATATGTTCTAACCTTTTCGATTTAATCA
ATTGAACTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCACT

Last Updated on 06.12.2018 16:11