Sequences from confirmation amplicon


Confirmation sequences for line 216B09 and hit At3g50230 are shown.
Line ID 216B09
Locus At3g50230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18622046
Primers used for PCR EP13 - 8474, show primer details
   
Sequence Name 20-K102174-0022-216-B09C-8409
GenBank Accession LN495155 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102174-0022-216-B09C-8409
TCGGACGNAGCCATTTACATTGAAGAAACAGAGGAGAAGAAGATGAAGAACATTACCATG
AATAAGATTGAAGAGACTTCCATTAGGTTGGTACTCATAGATGACAAGTCTCTCTCCATT
TGACTGGAAATAAGCTTTAACCGGAACAAGATTCGGATGCTTAAGTCCACCAACTATCTC
CATCTGATTCTCAAACTCTAAATCACTAGTAATCGCTGTTTTCGACGGAGCAAATCTCTT
CACCGTCACGATCATTTGATTAACCATCACCGCTTTATACGTTGTACCTACTGATCCTCT
TCCTAAAAGCTCTGCTGAAGCTCTCATCAACTGGTCAACTGTGTAC
   
Sequence Name 20-K102178-0022-216-B09C-EP13
GenBank Accession LN495156 [GenBank]
Sequencing primer gene specific primer, EP13: 5'-TGTACACAGTTGACCAGTTGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102178-0022-216-B09C-EP13
TAGGTACACGTATAAAGCGGTGATGGTTAATCAATGATCGTGACGGTGAAGAGATTTGCT
CCGTCGAAAACAGCGATTACTAGTGATTTAGAGTTTGAGAATCAGATGGAGATAGTTGGT
GGACTTAAGCATCCGAATCTTGTTCCGGTTAAAGCTTATTTCCAGTCAAATGGAGAGAGA
CTTGTCATCTATGAGTACCAACCTAATGGAAGTCTCTTCAATCTTATTCATGGTAATGTT
CTTCATCTTCTTCTCCTCTGTTTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11