Sequences from confirmation amplicon


Confirmation sequences for line 216D03 and hit At2g01480 are shown.
Line ID 216D03
Locus At2g01480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:217431
Primers used for PCR AH22 - 8474, show primer details
   
Sequence Name 10-K105564-0022-216-D03C-AH22
GenBank Accession LN490652 [GenBank]
Sequencing primer gene specific primer, AH22: 5'-GCATAAAGCTCCCTTCACAGATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105564-0022-216-D03C-AH22
GGCGTTATTCTTCTTATTTCGCCACCGTCCTCCGCCGCCGTGGTGGACTTATATCTCTGC
GTTTCACGACCTGCCGCCACCGCCGGCGATAGGTTTCAATGATGAATTAAGGCGTTGGCC
AGAGAGAGAAGAGAGATTGAGATCTGTCGATATGCTAGCGAAGAATCGGCTGCCGGGAAG
CGGCCACACTACTCCGTCTCCTCCGGCATCTCCTCGCCGTTCTCCTCGTTATCGTCACGG
TCGGTCTAAAGCCGCCGCCGGTAGTAGATTTCCTACGGTTCAACCAAGTCGGACCCTCGC
TCATCGCCTCTCGTGGATCCTCTTATCGGTTCTTCTCCGTCGTCAGGGGATTTTCTTATT
CGCTCCTCTAATCTATATCTCTTGTATGCTGCTTTACATGGGAACCGTCTCCTTCGACGT
TGTTCCAATCATTCAGCGTCGACCTCCTCCAGGATCCGTTTACAAAAGCCCTCAGGTTTA
CGCCAAGCTCCGTCCTGAGATGGACGCCGATAATTCCACCGCTGATGCCGTGAGTTCTCT
TTTTCCAGCTCCAACATTGTCTTACATAGAGATTTTGATTCTTAGTGAATCCTTAGCAAT
TTCCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 58-K105565w-0022-216-D03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K105565w-0022-216-D03C-8409
GCCATTTCATGGAAATTGCTAAGGATTCACTAAGAATCAAAATCTCTATGTAAGACAATG
TTGGAGCTGGAAAAAGAGAACTCACGGCATCAGCGGTGGAATTATCGGCGTCCATCTCAG
GACGGAGCTTGGCGTAAACCTGAGGGCTTTTGTAAACGGATCCTGGAGGAGGTCGACGCT
GAATGATTGGAACAACGTCGAAGGAGACGGTTCCCATGTAAAGCAGCATACAAGAGATAT
AGATTAGAGGAGCGAATAAGAAAATCCCCTGACGACGGAGAAGAACCGATAAGAGGATCC
ACGAGAGGCGATGAGCGAGGGTCCGACTTGGTTGAACCGTAGGAAATCTACTACCGGCGG
CGGCTTTAGACCGACCGTGACGATNACGAGGAGAACGGCGAGGAGATGCCGGAGGAGACG
GAGTAGTGTGGCCGCTTCCCGGCAGCCGATTCTTCGCTAGCATATCGACAGATCTCAATC
TCTCTTCTCTCTCTGGCCAACGCCTTAATTCATCATTGAAACCTATCGCCGGCGGTGGCG
GCAGGTCGTGAAACGCAGAGATATAAGTCCACCACGGCGGCGGAGGACGGTGGCGAAATA
AGAAGAATAAACGCCGGAAGAGAGAGAAAGAGAGTGTATGTTCTGTGA

Last Updated on 06.12.2018 16:11