Sequences from confirmation amplicon


Confirmation sequences for line 216D07 and hit At5g23670 are shown.
Line ID 216D07
Locus At5g23670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7983815
Primers used for PCR MC01 - 8474, show primer details
   
Sequence Name 19-K027154-022-216-D07C-8409
GenBank Accession LN494613 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K027154-022-216-D07C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATGTAGCAATATAGCCACCAC
AAGACCCAAAAGATTTTGTGAAGGTTCCCATCATTACGTCCACATCAGCTGTGTCAACTC
CCAGGAGTTCACAAATACCCTTTCCTGTTTTACCAATTGCCCCAATGCTGTGAGCTTCAT
CCAAATAAACATATGCCTGAAACGAAGATAGAAGATAAGTTAGGTGTATCTTTTCACGCA
ACAAAAAACTCCCAATAGTTTAGAAAATATGGCATTTCAAAGGGTAATTTTAGTCAATAG
AGTTCCTTAATTTATCGCTTATATTGAATGTGGCACATAGTGAACCCAGTTACTTTTTAA
GGGGTATTTGCACACACAAAATAGTTTCAGTTATCAACCAATTCTCATAATCAGTAATTC
AGGAATATTGTCTCACCTTATACTTCTTGCATATGGCCACAACTTCCGGTAGATGACAAA
TCTCCCCTTCCATGCTGTAAATACCTTCAACAACAACAATAATTTTCT
   
Sequence Name 23-K027154-022-216-D07C-MC01
GenBank Accession LN496416 [GenBank]
Sequencing primer gene specific primer, MC01: 5'-ATCGACCATGGAAGAAAATTATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K027154-022-216-D07C-MC01
TTGTTGAAGTATTTACAGCATGGAAGGGGAGATTTGTCATCTACCGGAAGTTGTGGCCAT
ATGCAAGAAGTATAAGGTGAGACAATATTCCTGAATTACTGATTATGAGAATTGGTTGAT
AACTGAAACTATTTTGTGTGTGCAAATACCCCTTAAAAAGTAACTGGGTTCACTATGTGC
CACATTCAATATAAGCGATAAATTAAGGAACTCTATTGACTAAAATTACCCTTTGAAATG
CCATATTTTCTAAACTATTGGGAGTTTTTTGTTGCGTGAAAAGATACACCTAACTTATCT
TCTATCTTCGTTTCAGGCATATGTTTATTTGGATGAAGCTCACAGCATTGGGGCAATTGG
TAAAACAGGAAAGGGTATTTGTGAACTCCTGGGAGTTGACACAGCTGATGTGGACGTAAT
GATGGGAACCTTCACAAAATCTTTTGGGTCTTGTGGTGGCTATATTGCTACATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACA
Primers used for PCR MC02 - 8474, show primer details
   
Sequence Name 51-K027155-022-216-D07C-8409
GenBank Accession LN507381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K027155-022-216-D07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCGGACATGAAGCCATTTA
CTCATACTCAGATTGTCATATATTTCCGAAAATGCTACATTTGTTTTTATTTACTGATCT
TGCAAATCTCCTGCAGTCTTGTTTTGGTAAATCAAGTTTCTGACCTTTTAGTTTAGCTCA
AAAAGAATATTGATAGCCGTAACTTTCACTCAAATATAAACCATTAACCAAAAAAACGCT
CACTCAAAAGTCAAAACTTATTTTGAACCTGCGAAATGACGATTAGTATATATATATAAA
GGCCTTCAGTATCTATACGACATACCATGTGATACTTAGGTAAGCTGTACACTTCTTAAA
TTCAACAAAAGTATAATCAATTTATTATTTTTATTCAACTTCTATAGGAGCTCATCCAAT
ATTTGAAGCATCAATGCCCAGCTCATCTTTACGCAACATCCATACCAACTCCTTCCGCAC
AACAAATTATATCTGCCATTAAAGTTATTCTGGGAGAGGATGGTTCAAATCGAGGTACTC
GATTTTTTTTCTCACCGTGTCATTAATTGGCCTCATTTGGGTCACTGAGCACAATC
   
Sequence Name 55-K027155-022-216-D07C-MC02
GenBank Accession LN508690 [GenBank]
Sequencing primer gene specific primer, MC02: 5'-TCATGACCTGATTAGATTGTGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K027155-022-216-D07C-MC02
CCAAATGAGGCCAATTAATGACACGGTGAGAAAAAAAATCGAGTACCTCGATTTGAACCA
TCCTCTCCCAGAATAACTTTAATGGCAGATATAATTTGTTGTGCGGAAGGAGTTGGTATG
GATGTTGCGTAAAGATGAGCTGGGCATTGATGCTTCAAATATTGGATGAGCTCCTATAGA
AGTTGAATAAAAATAATAAATTGATTATACTTTTGTTGAATTTAAGAAGTGTACAGCTTA
CCTAAGTATCACATGGTATGTCGTATAGATACTGAAGGCCTTTATATATATATACTAATC
GTCATTTCGCAGGTTCAAAATAAGTTTTGACTTTTGAGTGAGCGTTTTTTTGGTTAATGG
TTTATATTTGAGTGAAAGTTACGGCTATCAATATTCTTTTTGAGCTAAACTAAAAGGTCA
GAAACTTGATTTACCAAAACAAGACTGCAGGAGATTTGCAAGATCAGTAAATAAAAAC

Last Updated on 06.12.2018 16:11