Sequences from confirmation amplicon


Confirmation sequences for line 216D09 and hit At4g21160 are shown.
Line ID 216D09
Locus At4g21160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11285129
Primers used for PCR 03im - 8474, show primer details
   
Sequence Name 53-K113020-0022-216-D09C-03im
GenBank Accession LN508207 [GenBank]
Sequencing primer gene specific primer, 03im: 5'-GACTGATTTATCCACCTTGTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113020-0022-216-D09C-03im
CTTTACTCATCTGATACCGAGTCTGCGCTGATTGTGGCGCTCCAGATCCTAAGTGGGCGT
AAGTTTTGCCATTTTTATGTTTTGTTCCAGTAAGCATTTTATGCTTCTCTAAGCAGTTTG
ATTGAATGTTATGATCTTGTTTCCCTACTTGTGCATTTTTTGGTTTGTTTCGTTTTTCGT
AAACCCATAAAACTCTTGGTTGCTTATTACAGGTCAGCTAATATTGGAGTGTTTATATGC
TTAAAATGTTGTGGTGTCCACAGAAGCCTTGGCAGTCATATCTCAAAAATGGCTGATCCA
ATGAGTAAATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 21-K113019-0022-216-D09C-8409
GenBank Accession LN495738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K113019-0022-216-D09C-8409
ATTTACATTGAATATATCCTGGAAATTTACTCATTGGATCAGCCATTTTTGAGATATGAC
TGCCAAGGCTTCTGTGGACACCACAACATTTTAAGCATATAAACACTCCAATATTAGCTG
ACCTGTAATAAGCAACCAAGAGTTTTATGGGTTTACGAAAAACGAAACAAACCAAAAAAT
GCACAAGTAGGGAAACAAGATCATAACATTCAATCAAACTGCTTAGAGAAGCATAAAATG
CTTACTGGAACAAAACATAAAAATGGCAAAACTTACGCCCACTTAGGATCTGGAGCGCCA
CAATCAGCGCAGACTCGGTTATCAGATTGAGTTAAAAGATCCCTTATTCTCCTTTTACCT
GTGAACCAACAAGGTGGAAAAAAATCAGT

Last Updated on 06.12.2018 16:11