Sequences from confirmation amplicon


Confirmation sequences for line 216G08 and hit At1g60410 are shown.
Line ID 216G08
Locus At1g60410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22260007
Primers used for PCR KH10 - 8474, show primer details
   
Sequence Name 04-K103100-0022-216-G08C-KH10
GenBank Accession LN487874 [GenBank]
Sequencing primer gene specific primer, KH10: 5'-TATGGTGGATGTTGATGTAGCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103100-0022-216-G08C-KH10
CTTTAGTGAATTTGATTCTCTACTGTGTTGTCTTTGATCATCCAAGATCAAAATCGGTTT
CTTTACCTAGTGTGAAGAAAATGTATTTTGAGGGGGTCAAGTTTGATGGTGATTCTGTTC
TGGAGACTCTCATATCGCATAGTCCGGTTCTTGAGGAGTTAACCGTGATTCCGCATCCGG
AGGATTATTTGGAAGTCATATGTGTTCGTTCTCAATCACTGGAGAGCTTTAGACTTGAGT
CTAAGCGTTTTGAGTGTGACAATCCCAAAGTTGAAATCGATTCTCCAAGCCTTGAGTTTA
TGAGTATCTGTGATAAGAAACCTGAAAGCCTTAAAATACATAGAATTGGTCCTTTTGCGG
AGGTAACCGTTGATGTTGAGTTTGATGTTGAGGATGATGATCCATTGGAGATATCTAAGA
TACGTAAGTTTCTCGTCGGGTTATCCACATTTCACGAACTGACCTAAGAAAAAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAAGTAGATG
TCCGCACGGTTTATT
   
Sequence Name 04-K103101-0022-216-G08C-8409
GenBank Accession LN487875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103101-0022-216-G08C-8409
CATTTACAATTGAATATATCCTTTTTCTTAGGTCAGTTCGTGAAATGTGGATAACCCGAC
GAGAAACTTACGTATCTTAGATATCTCCAATGGATCATCATCCTCAACATCAAACTCAAC
ATCAACGGTTACCTCCGCAAAAGGACCAATTCTATGTATTTTAAGGCTTTCAGGTTTCTT
ATCACAGATACTCATAAACTCAAGGCTTGGAGAATCGATTTCAACTTTGGGATTGTCACA
CTCAAAACGCTTAGACTCAAGTCTAAAGCTCTCCAGTGATTGAGAACGAACACATATGAC
TTCCAAATAATCCTCCGGATGCGGAATCACGGTTAACTCCTCAAGAACCGGACTATGCGA
TATGAGAGTCTCCAGAACAGAATCACCATCAAACTTGACCCCCTCAAAATACATTTTCTT
CACACTAGGTAAAGAAACCGATTTTGATCTTGGATGATCAAAGACAACACAGTAGAGAAT
CAAATTCACTAAAGTAGCACATGAATAAAGCGAGGGAGGCATCCTAACTAATGCTACATC
AACATCCACCAT

Last Updated on 06.12.2018 16:11