Sequences from confirmation amplicon


Confirmation sequences for line 217A04 and hit At1g05300 are shown.
Line ID 217A04
Locus At1g05300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1547048
Primers used for PCR GC46 - 8474, show primer details
   
Sequence Name 15-K033539-022-217-A04C-8409
GenBank Accession LN492870 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K033539-022-217-A04C-8409
ATTTTATACATGTAGACGTGACCTCTGTTTTCTAAACTTTCTTAAAGGAAAAAAAGTTAA
ATATATATGACCATGTTGAGAATATGTCACTGAATTTTCATTTGCCTATTGTTTATTTTT
TTCTTCGTCAACTTGTTTTTGTTTTTCTTAAGTAATTTTGTCGTCAACTTCCATATTTAG
CATTTTGTAGATTATATACTCTTATTTTTTTTGGTTGATTATGTACTAGTCTGGTTTGAC
ATAATAAAAAGAACTATATTTTTTAATTGAATTATATAAAACTTACATATATCATTAATA
TATATATCATATAGTTTTTCTTTATGTAAATTAAAATTTTAAGGTCCATGGTTTTTTGGT
CCTGGCGGGTAAAAGATTCTTGGGAGAAATTTGTCCACATATTAATTATAGGGTCCCCAA
AAGATAAAGCCAGAAAAACCAACTCTTGCTCTTAATAGAGTTACTGGTAATAGTCATACG
GGTTGTATCATATAAGTATAAGTTTGAAGTGAAAGTGAGTCAACAAAATGTCGGAAAACC
TAAAATTAGATCAAGATGAAATCTTTTCTTGTGTATGAAACTGTGCATAAAAATAGAGAT
ACAATAATAAGTGATGGGTCCATATGAGG
   
Sequence Name 15-K033539-022-217-A04C-GC46
GenBank Accession LN492871 [GenBank]
Sequencing primer gene specific primer, GC46: 5'-CCTCATATGGACCCATCACTTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K033539-022-217-A04C-GC46
GTTTCTACCAGAAAAGATTTCATCTTGATCTAATTTTAGGTTTTCCGACATTTTGTTGAC
TCACTTTCACTTCAAACTTATACTTATATGATACAACCCGTATGACTATTACCAGTAACT
CTATTAAGAGCAAGAGTTGGTTTTTCTGGCTTTATCTTTTGGGGACCCTATAATTAATAT
GTGGACAAATTTCTCCCAAGAATCTTTTACCCGCCAGGACCAAAAAACCATGGACCTTAA
AATTTTAATTTACATAAAGAAAAACTATATGATATATATATTAATGATATATGTAAGTTT
TATATAATTCAATTAAAAAATATAGTTCTTTTTATTATGTCAAACCAGACTAGTACATAA
TCAACCAAAAAAAATAAGAGTATATAATCTACAAAATGCTAAATATGGAAGTTGACGACA
AAATTACTTAAGAAAAACAAAAACAAGTTGACGAAGAAAAAAATAAACAATAGGCAAATG
AAAATTCAGTGACATATTCTCAACATGGTCATATATATTTAACTTTTTTTCCTTTAAGAA
AGTTTAGAAAACAGAGGTCACGTCTACATGTATAAAATCTACATGTCCGGAAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11