Sequences from confirmation amplicon


Confirmation sequences for line 217A09 and hit At5g60410 are shown.
Line ID 217A09
Locus At5g60410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24297142
Primers used for PCR KR23 - 8474, show primer details
   
Sequence Name 27-K030468-022-217-A09C-8409
GenBank Accession LN498257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K030468-022-217-A09C-8409
GCATAGCACCTGCTGAAGAAATACTGTTACTCGGCTTTTCGTTTGTACTAATACTCCGAT
CCAAAACTTTTCAGTGCAAGCACAATGCAGAGTGTGGAGAGAACATTTCAAATCACAAGG
GCAGACAAGGACCTTTTGGCCAAACCAGAGTACGATGTTCAGGTTGGGTTTTGTAAACTA
CATTTAATATGTCAAGGTGTATCTACCTATACGTTTCTAACTGGAAATCAATTTTGTTTA
GGCTTGGTGTATGCTCTTGAATGATAAAGTTCTCTTTAGGATGCAGTGGCCTCAGTATGC
TGATCTGCAGGTCAATGGTATGACAATGACATCGACCAGAGTTTTA
   
Sequence Name 28-K030468-022-217-A09C-KR23
GenBank Accession LN498719 [GenBank]
Sequencing primer gene specific primer, KR23: 5'-GAAATGCTCAACCGACTAAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K030468-022-217-A09C-KR23
GGCCACTGCATCCTAAAGAGAAACTTTATCATTCAAGAGCATACACCAAGCCTAAACAAA
ATCGATTTCCAGTTAGAAAACGTATAGGTAGATCACACCTCGACATATTAAATCGTAGTT
TACAAAACCCAACCTGAACATCGTACTCTGGTTTGGCCAAAAGGTCCTCGTCTGCCCTTG
TGATTTGAAATGTTCTCTCCACACTCTGCATTGTGCTTGCACTGAAAAGTTTTGGATCGG
AGTATTAGTACAAACAAAAGCCAGTAACAGTATTTCTTCAGCAGGTGCTATGCATATAGC
CAGGAAACAATTGTCATTCTTGAAACTAGAATGAATGTCTTATACCAGTTCCAAGTAGAA
AATTTAAAGTCAAAGTGACTTATAAAGTACCAATTCACACAATAATGGCACTGAAAAAGA
AAAATTTATAGATGGCTAAGAGTAAATGGCTTCATGTCCGGGATATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11