Sequences from confirmation amplicon


Confirmation sequences for line 217A12 and hit At5g01160 are shown.
Line ID 217A12
Locus At5g01160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:54573
Primers used for PCR 07nh - 8474, show primer details
   
Sequence Name 36-K114626-0022-217-A12C-07nh
GenBank Accession LN502326 [GenBank]
Sequencing primer gene specific primer, 07nh: 5'-CAGGAAAGACCTAAGACAATGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114626-0022-217-A12C-07nh
TCTTGATTGTTTGATCTTCTGAATCCGCTCATCACATCTGTGCAGTAATCACACACACAA
GAGACAACAAGATTTCAGTCTGATAATCGCTACGCCTAGTAAATCTTAAAATGTCTCTGT
AGATTACTAAACAATCTAGAATATCTCTATCAAAGCTTCTAATGAAATTCGAAGCCTAGT
TCAAACTATGATAATCCTGCGTCTCTGTAGATTACCAAACAATCTAAAGTATCTCTATCA
AAGCTTCTAATGAAATTCGATGAGAGTTAAAGATGGTTACAGATAGCAGATGGAATCACT
GCGAGCACATTCAAGGCAAAAGGCATGATCACAAGGAATCTGCAAAACCAAAACAAAACA
AAAAAAGTTTCAATCACATATAGAAGAAGAGCCCCCTTAAAAAAACTCTTTAAGGCAAAT
CAATATAGCCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATAT
CCG
   
Sequence Name 36-K114625-0022-217-A12C-8409
GenBank Accession LN502325 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114625-0022-217-A12C-8409
TTGATTTGCCTTAAGAGTTTTTTTAGGGGGCTCTTCTTCTATATGTGATTGAAACTTTTT
TTGTTTTGTTTTGGTTTTGCAGATTCCTTGTGATCATGCCTTTTGCCTTGAATGTGCTCG
CAGTGATTCCATCTGCTATCTGTAACCATCTTTAACTCTCATCGAATTTCATTAGAAGCT
TTGATAGAGATACTTTAGATTGTTTGGTAATCTACAGAGACGCAGGATTATCATAGTTTG
AACTAGGCTTCGAATTTCATTAGAAGCTTTGATAGAGATATTCTAGATTGTTTAGTAATC
TACAGAGACATTTTAAGATTTACTAGGCGTAGCGATTATCAGACTGAAATCTTGTTGTCT
CTTGTGTGTGTGATTACTGCACAGATGTGATGAGCGGATTCAGAAGATTCAAACAATCAA
GATGATGGAAGGGATCTTCATCTGTGCAGCTCCTCATTGTCTTAGTCTTTTCCT

Last Updated on 06.12.2018 16:11