Sequences from confirmation amplicon


Confirmation sequences for line 217B05 and hit At4g24570 are shown.
Line ID 217B05
Locus At4g24570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12687023
Primers used for PCR LB20 - 8474, show primer details
   
Sequence Name 31-K115234-0022-217-B05C-LB20
GenBank Accession LN500286 [GenBank]
Sequencing primer gene specific primer, LB20: 5'-TTGTCTAACTCATAGCAACAAGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K115234-0022-217-B05C-LB20
ACATGGGCTCTGCCCGCGAGAGTTCGAATCTCTCAGGCGACGTTTCTTTTGTTTTCGGCC
ATAAAGGAAAAAGCCCAATTAACACGTCTCGCTTATAAGCCCATAAAGCAAACAATGGGC
TGTCTCTGTCTCACTCACACACGCGTTTTCCTACTTTTTGACTATTTTTATAACCGGCGG
GTCTGACTTAATTAGGGTTTTCTTTAATAATCAGACACTCTCTCACTCGTTTCGTCAACA
TTGAACACAGACAAAACCGCGTCACAAAACAAAACTCGCTACAAATAAATACCTTTGTTT
CCCTCTTCTTCTCCTTCACTCACAACATCTCAATTTCATTCTCTCTTCTCTCTCCAATTT
CACAACAATGGGAGTCAAAAGTTTCGTTGAAGGTGGGATTGCCTCTGTAATCGCCGGTTG
CTCTACTCACCCTCTCGATCTAATCAAGGTTCGTCTTCAGCTTCACGGTGAAGCACCTTC
CACCACCACCGTCACTCTCCTCCGTCCAGCTCTCGCTTTCCCCAATTCTTCTCCTGCAGC
TTTCCTGGAAACGACTTCTTCAGTCCCCAAAGTAGGACCGATCTCACTCGGAATCAACAT
AGTCAAATCGGAAGGCGCCGCCGCGTTATTCTCAGGAGTCTCCGCTACACTTCTCCGTCA
GACGTTATATTCCACCACCAGGATGGGTCTATACGAAGTGCTTAAGAACAAATGGACTGA
TCCTGAGTCAGGGAAGTTGAATCTGAGTAGGAAGATCGGTGCAGGGCTAGTCGCTGGTGG
AATCGGAGCCGCCGTTGGAAATCCAGCTGACGTGGCGATGGTTAGGATGCAAGCTGACGG
GAGGTGTGATGCAAGCTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCNGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAGAGTAATTACCAA
TTTTTTTTCAATCAAAAATGTAG
   
Sequence Name 07-K115233-0022-217-B05C-8409
GenBank Accession LN489399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115233-0022-217-B05C-8409
TCGGACTGAGCATTTCATTGAATAATCCTGTCAGCTTGCATCACACCTCCCGTCAGCTTG
CATCCTAACCATCGCCACGTCAGCTGGATTTCCAACGGCGGCTCCGATTCCACCAGCGAC
TAGCCCTGCACCGATCTTCCTACTCAGATTCAACTTCCCTGACTCAGGATCAGTCCATTT
GTTCTTAAGCACTTCGTATAGACCCATCCTGGTGGTGGAATATAACGTCTGACGGAGAAG
TGTAGCGGAGACTCCTGAGAATAACGCGGCGGCGCCTTCCGATTTGACTATGTTGATTCC
GAGTGAGATCGGTCCTACTTTGGGGACTGAAGAAGTCGTTTCCAGGAAAGCTGCAGGAGA
AGAATTGGGGAAAGCGAGAGCTGGACGGAGGAGAGTGACGGTGGTGGTGGAAGGTGCTTC
ACCGTGAAGCTGAAGACGAACCTTGATTAGATCGAGAGGGTGAGTAGAGCAACCGGCGAT
TACAGAGGCAATCCCACCTTCAACGAAACTTTTGACTCCCATTGTTGTGAAATTGGAGAG
AGAAGAGAGAATGAAATTGAGATGTTGTGAGTGAAGGAGAAGAAGAGGGAAACAAAGGTA
TTTATTTGTAGCGAGTTTTGTTTTGTGACGCGGTTTTGTCTGTGTTCAATGTTGACGAAA
CGAGTGAGAGAGTGTCTGATTATTAAAGAAAACCCTAATTAAGTCAGACCCGCCGGTTAT
AAAAATAGTCAAAAAGTAGGAAAACGCGTGTGTGAGTGAGACAGAGACAGCCCATTGTTT
GCTTTATGGGCTTATAAGCGAGACGTGTTAATTGGGCTTTTTCCTTTATGGCCGAAAACA
AAAGAAACGTCGCCTGAGAGATTCGAACTCTCGCGGGCAGAGCCCATGTACTTAGCAGGC
ACACGCCTTAACCACTCGGCCAAAGCGACTTGTTGCTAT

Last Updated on 06.12.2018 16:11