Sequences from confirmation amplicon


Confirmation sequences for line 218A04 and hit At5g37680 are shown.
Line ID 218A04
Locus At5g37680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14970583
Primers used for PCR JU25 - 8474, show primer details
   
Sequence Name 31-K023370w-022-218-A04C-JU25
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, JU25: 5'-GAATCGAACTAGGGCTACGAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K023370w-022-218-A04C-JU25
CGATTTTGAATCTCCAAATCCAGAACTCGAGTGTTAAATCTTCGAAAAGATCAAATCTTT
GGAGATGGGTCTTTGGGATTCACTTCTTAATTGGCTCCGGAGGTAAACAAATTTCTAACT
TTCATTTTGTTTTGCTGATTAAGTTGGTTTATTAGTTGTAGTCCTTTTGTTCTTCCATTG
AAGTAGTTTAGAGTTATAGTTCTGGGTTTAGTAAAAACGTTGCCAAAAACCAATTTTTCT
CACAAAGAGTCAACAATTATTGTTTCAAAATCTCAAACTTCTCTTGAGTTTCTTGGGCTG
TATCTTTTATTGTTACATTATATGAATTGGTTCGCTGAGAAATCTACCGTTATTTCTATG
TTCTTGTGTATTAATTCTCTTGACTTGATTGTCATTTTAATCCATTTATAGTAGTGATGC
TTTATGTGATGTTATAGAATGGTTAAAAGAGTAGGTTATAGTTGGTTAGTAATGTATTGT
TGTTTTTGGTTATACTTATGTTTTTAGTGTATAGCAGAATCACGTTTTGGTAACACTTAG
ATTTTGATTCTGTGTTTGCTTATATTTGTTGTGTCAGCTTTTACACT
   
Sequence Name 27-K023370w-022-218-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K023370w-022-218-A04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTAAGTGTAAAAGCTGAC
ACAACAAATATAAGCAAACACAGAATCAAAATCTAAGTGTTACCAAAACGTGATTCTGCT
ATACACTAAAAACATAAGTATAACCAAAAACAACAATACATTACTAACCAACTATAACCT
ACTCTTTTAACCATTCTATAACATCACATAAAGCATCACTACTATAAATGGATTAAAATG
ACAATCAAGTCAAGAGAATTAATACACAAGAACATAGAAATAACGGTAGATTTCTCAGCG
AACCAATTCATATAATGTAACAATAAAAGATACAGCCCAAGAAACTCAAGAGAAGTTTGA
GATTTTGAAACAATAATTGTTGACTCTTTGTGAGAAAAATTGGTTTTTGGCAACGTTTTT
ACTAAACCCAGAACTATAACTCTAAACTACTTCAATGGAAGAACAAAAGGACTACAACTA
ATAAACCAACTTAATCAGCAAAACAAAATGAAAGTTAGAAATTTGTTTACCTCCGGAGCC
AATTAAGAAGTGAATCCCAAAGACCCATCTCCAAAGATTTGATCTTTTCGAAGATTTAAC
ACTCGAGTTCTGGATTTGGAGATTCAAAATCGAAATATTTCGT

Last Updated on 06.12.2018 16:11