Sequences from confirmation amplicon


Confirmation sequences for line 218B05 and hit At5g15230 are shown.
Line ID 218B05
Locus At5g15230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4945366
Primers used for PCR MR64 - 8474, show primer details
   
Sequence Name 51-K017281-022-218-B05C-MR64
GenBank Accession LN507341 [GenBank]
Sequencing primer gene specific primer, MR64: 5'-TATAAAAAGGGAACGAAGGGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K017281-022-218-B05C-MR64
AATTTTTCAAGGGCATTTTGGTCCACCCTCTTGAGTTTTCCAGTTGTTGTAGCAGGAGCA
AACTTGTTTGTTCCCATAGTAACCCGGAGGCACACAGAGACACTTCCTGCAGCATTTGTT
GCAGAACGTAATGCAAGCCTTGTGGTACTGTGTCTTTTTACACCTCCTATCACATTCCGA
TGGGCATTCTGGTTGTTTCAAATACAAACAATATCAAAATGTTAACACTCAAAAATTTTT
TATTACTAATAAAAACAACAGAATCTAGTGGAAATTCATTAGCTTTTTTTTTTACTGTTT
TGTGGGTCTCAAGATAGTGGGTTGCTTTGACTGTATGACCATATTAAGGGGCAATTTCGT
CAATATCTAATATATTTTGAGTCTACGGATCTGACCAAACAGTCCACTTAACACTTCACT
TTCAACTTATTTTCTGCAACTGAAATGCTTATTTTTTTTTATTATAACACAATAAAAGAT
TTCTTGAATTGTTTATTTTTCGCCGATTATGAGATTAAAAAAAAATTGTTTAAAAATAGC
TGTGAAAAAAAAACTTACGGGTACGTTTCAGGCTTCCTGGTCCATAACGTTTCTGAAAAT
TAACCAAAAAAGGTAACTAATTAATCACTTTTCGTTAAGTTACGAGATAACAATAGGTAT
AAAATAGAAAACCTCTTGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
   
Sequence Name 52-K017281-022-218-B05C-8409
GenBank Accession LN507665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K017281-022-218-B05C-8409
GTAGATTTCCCGGACATGAGGTTTTCTATTTTATACCTATTGTTATCTCGTAACTTAACG
AAAAGTGATTAATTAGTTACCTTTTTTGGTTAATTTTCAGAAACGTTATGGACCAGGAAG
CCTGAAACGTACCCGTAAGTTTTTTCTTCACAGCTATTCTTAAACAATTTTTTTTTAATC
TCATAATCGACGAAAAATAAACAATTCAAGAAATCTTTTATTGTGTTATAATAAAAAAAA
ATAAGCATTTCAGTTGCAGAAAATAAGTTGAAAGTGAAGTGTTAAGTGGACTGTTTGGTC
AGATCCGTAGACTCAAAATATATTAGATATTGACGAAATTGCCCCTTAATATGGTCATAC
AGTCAAAGCAACCCACTATCTTGAGACCCACAAAACAGTAAAAAAAAAAGCTAATGAATT
TCCACTAAATTCTGTTGTTTTTATTAGAAATAAAAAATTTTTGAGGGTTAACATTTTGAT
ATTGTTTGTATTTGAAACAACCAAAATGCCCATCGGAATGTGATAGGAGGTGTAAAAAGA
CACAGTACCACAAGGCTTGCATTACGTTCTGCAACAAATGCTGCAGGAAGTGTCTCTGTG
TGCCTCCGGGTTACTATGGGAACAAACAAGTTTGCTCCTGCTACAACAACTGGAAAACTC
AAGAGGGTGGACCAAAATGCCCTTGAAAAAATCTCC

Last Updated on 06.12.2018 16:11