Sequences from confirmation amplicon


Confirmation sequences for line 219B04 and hit At1g20670 are shown.
Line ID 219B04
Locus At1g20670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7166614
Primers used for PCR 02kz - 8474, show primer details
   
Sequence Name 36-K113872-0022-219-B04C-02kz
GenBank Accession LN502303 [GenBank]
Sequencing primer gene specific primer, 02kz: 5'-TGAATTGTATTCCATTGCATTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K113872-0022-219-B04C-02kz
AAAGACAGGAGGCGAGAAGCTATATATCAGAGAGGGAAAAAAGTTGTTCAAACACAAATC
TTCTCACAAATAACATATATTCAAGCGGGGAGACAATTTTGAGTCTAAACTGATCAAAGG
TTAATTTTGAGTTAAACAGAAGTGTTGAAATAGCCTTAAATCTGCACCCTACACTACATG
TATCTTCACTGGTTAGCACTTAGCAGTTATAAAGCCTTACAAATCTTATAACTGAAAGAA
AACGCACACAAACAACATCATAAAGCAATGGTTCTCCGTAACAAAGTATTGAATTGAAGA
TGCCAAGTAACAAAACTTTCTGTAGCTAGCAATCAAATGAGGAACCAACTCGAACGCATC
AAAAAGGAGGAGTAGAAAGACATACCTCAAATTGTTCTAAAGTGGAATATGCTCCAGAGT
CTAATTTGTTCCGCAATGTGCTAAAATCCATTGGATTCTTAATAATCTCAAAATAATCAG
GAAGCTGTAAAAAAGACACAGATAAGACAAACAGAATCTCGTAACATTAACAAGATGAAA
ACATCAACAAAAAGAGACAATCACCTCCTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 10-K113871-0022-219-B04C-8409
GenBank Accession LN490742 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K113871-0022-219-B04C-8409
TCATTGAATTATCCTGAGGAGGTGATTGTCTCTTTTTGTTGATGTTTTCATCTTGTTAAT
GTTACGAGATTCTGTTTGTCTTATCTGTGTCTTTTTTACAGCTTCCTGATTATTTTGAGA
TTATTAAGAATCCAATGGATTTTAGCACATTGCGGAACAAATTAGACTCTGGAGCATATT
CCACTTTAGAACAATTTGAGGTATGTCTTTCTACTCCTCCTTTTTGATGCGTTCGAGTTG
GTTCCTCATTTGATTGCTAGCTACAGAAAGTTTTGTTACTTGGCATCTTCAATTCAATAC
TTTGTTACGGAGAACCATTGCTTTATGATGTTGTTTGTGTGCGTTTTCTTTCAGTTATAA
GATTTGTAAGGCTTTATAACTGCTAAGTGCTAACCAGTGAAGATACATGTAGTGTAGGGT
GCAGATTTAAGGCTATTTCAACACTTCTGTTTAACTCAAAATTAACCTTTGATCAGTTTA
GACTCAAAATTGTCTCCCCGCTTGAATATATGTTATTTGTGAGAAGATTTGTGTTTGAAC
AACTTTTTTCCCTCTCTGATATATTAGCTTCTCGCCTCCTGTTCTTTTTGCAGAGAGATG
TGTTTTTAATATGTACAAATGCAATGG

Last Updated on 06.12.2018 16:11