Sequences from confirmation amplicon


Confirmation sequences for line 219H11 and hit At3g19960 are shown.
Line ID 219H11
Locus At3g19960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6956323
Primers used for PCR MB11 - 8474, show primer details
   
Sequence Name 16-K023359-022-219-H11C-8409
GenBank Accession LN493253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K023359-022-219-H11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGTCCTCAACAGCTA
AGCTTTTCTTTGCTATGGAAAGACTGGACTGCAACGACCTCATTTGCTTTTGCCAGATTT
CTTCCATTGATTTCATCTTTGTCTCATACTCTGACCACCGGTTCTCATATTGTTGAAGCC
TCTGCTGAAGAATGTCGTTCTCTTCTTCCTTTTCACGCAGAGCAGCTTCCGCTTTAAGTA
CCCTGCGTTGGAGCTCGGAAAGTACTGATGCTTTCACTAGCACCTCACCTAACTCATTTG
TCTGCGCAGTTGGTTAATGCCAAAGCAAAAATAGTTCAAAAGTTTATGAGATGAAAGAAA
ATAATTAGACACAGTGAGAAGAAAGGAATGTGATATATAACAATTATTTTACCTTGGCAC
CTCCAGATTTTAACCATCCAATATCCCCTGAACATCTTCTAACCAACCAACCACGAATTG
CTGCAAAAAGGTTATAAGAATTCAGTATCAACGTTGAATAAGCTTAAGCATTTTTCTTAA
TGAGAAGATAT
   
Sequence Name 17-K023359-022-219-H11C-MB11
GenBank Accession LN493688 [GenBank]
Sequencing primer gene specific primer, MB11: 5'-GCTTAGATGCTGCATATCTTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K023359-022-219-H11C-MB11
GAAAAAGCTTAAGCTTATTCAACGTTGATACTGAATTCTTATAACCTTTTTGCAGCAATT
CGTGGTTGGTTGGTTAGAAGATGTTCAGGGGATATTGGATGGTTAAAATCTGGAGGTGCC
AAGGTAAAATAATTGTTATATATCACATTCCTTTCTTCTCACTGTGTCTAATTATTTTCT
TTCATCTCATAAACTTTTGAACTATTTTTGCTTTGGCATTAACCAACTGCGCAGACAAAT
GAGTTAGGTGAGGTGCTAGTGAAAGCATCAGTACTTTCCGAGCTCCAACGCAGGGTACTT
AAAGCGGAAGCTGCTCTGCGTGAAAAGGAAGAAGAGAACGACATTCTTCAGCAGAGGCTT
CAACAATATGAGAACCGGTGGTCAGAGTATGAGACAAAGATGAAATCAATGGAAGAAATC
TGGCAAAAGCAAATGAGGTCGTTGCAGTCCAGTCTTTCCATAGCAAAGAAAAGCTTAGCT
GTTGAGGACTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA

Last Updated on 06.12.2018 16:11