Sequences from confirmation amplicon


Confirmation sequences for line 220E05 and hit At3g45030 are shown.
Line ID 220E05
Locus At3g45030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16471675
Primers used for PCR FA31 - 8474, show primer details
   
Sequence Name 17-K118658-0022-220-E05C-FA31
GenBank Accession LN494071 [GenBank]
Sequencing primer gene specific primer, FA31: 5'-AAATGCGTTGCACTAGTCTTAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118658-0022-220-E05C-FA31
TGAATACTGAGAAATGTTTTTGCTCTGTTGCTGCAAATGATATATGTAGTGTGCACTGAT
TTGGTTCGTGGAGCTCAGGACAAGACACTTATAGTCAAGGGACCAGTGAGAATGCCCACT
AAGGTTCTCGAGATCACTACCCCAATAGCTCCCTGTGGTGAAGGTATACTTCTGGTTCTC
TCATCTGTTATGTCTTCTAAAACACTAGAGCTTGAGTTTTGGGAACCGATCCTAAAGTGT
CACGTATTTCTATCTCACAAGATGTGATTGTGCTCAAGACTTCGAGTATCTTCTTCGGCT
TGGCCGATACTCTCTTGACTCTTGGTGTTGTGACCTGGAACCCATACATGGGACAGATGT
GAACTTAGGGTGCACTAGCGAGTGATTGATCTCTTCATCTCTCCAGAAGTGATTATTCGG
TTGTTCATGGCTACATGTCCGGGAAATCTACATGGATCAGCAAAGAATAGTATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 02-K118657-0022-220-E05C-8409
GenBank Accession LN487246 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118657-0022-220-E05C-8409
CATTTACATTGAATAATCACATCAGGAGAGCTGAAGAGATCAATCACTCGCTTGTGCACC
CTAAGTTCAAATCTGTCCCATGTATTGGTTCCTGTTACAACACCAATAGTTAAAAGAGTC
TCAGCCAATGTGAAGAAGATACTTGAAGTATTGAGCACAATCACATGTTCTAAGATCTAA
ATCCGCGACACTTTAGGAACGGTTCCCAAAACTCAAGCTCTAGTGTTTTTGAAGACATAA
AAGATGAGAGAACCAGAAGTATACCTTCACCACAGGGAGCTTTTCGGGTAGTGATCTTGA
GAACCTTAGTGGGCATTCTCACTGGTCCCTTGACTCTAAGTCTCTTGTCCTTAGCTCCAC
GAACCAAATCAGTGCACACTACATATATCATTTGCAGCAACAGAGCAAAAACATTTATTC
AGTATTCAATAATTGGAACATCTCTCAGATAGCCTCAAAATACTCTAATGTAACCTAAGA
CTAGTGCAACGCAA

Last Updated on 06.12.2018 16:11