Sequences from confirmation amplicon


Confirmation sequences for line 221C05 and hit At1g09470 are shown.
Line ID 221C05
Locus At1g09470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3056406
Primers used for PCR CP61 - 8474, show primer details
   
Sequence Name 59-K105140-0022-221-C05C-CP61
GenBank Accession LN509997 [GenBank]
Sequencing primer gene specific primer, CP61: 5'-CTCCAGTGAGCCGATATAATCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105140-0022-221-C05C-CP61
ATTTCTATCTTAGCCGTCAAAGTTTGTTGATTCTTTCAGAATTCTTTGGAGTATCTTCAT
TTAGCATGTTCCGCACCTCAGAGTTCTCCTTGGTCTTGACCACTGATACAGCCCGCATAA
TGTCACCTTCAACTTTCAAAAGCTCATCTCTTAGCTGCTTCTGTGAAGATTCTCTAACTC
GCAGCTCCTTTCTTAGATTCTCTAGCTGCTCACCAAGTCTAGTTACTTGGTCCTCATGCT
CTTTCAAAGAACCGGTCCTCTCATGCAATTGTTTCGAAAGCACTCTACAATGAGAATGTG
CTGATTCAGCTGACAAAGCACTTGCCTCTGCAGTTGCATGTGTTGCTGCTAGCTGTGATT
TGATATCAGCCAATTCCTTCACAAACTGCACAATAGGAGAACAGAAAAGTTGATAAGTTA
CGTCGGTAAAGGACGTGAGATCTCCTGAGTTTTGGCAGATTTAAGACCAAAGTCAGGTTC
AAAAGACAATGGAGGACCAGCAAAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 43-K105141-0022-221-C05C-8409
GenBank Accession LN504833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K105141-0022-221-C05C-8409
ATTTACAATTGAATATATCCTGACTTTGCTGGTCCTCCATTGTCTTTTGAACCTGACTTT
GGTCTTAAATCTGCCAAAACTCAGGAGATCTCACGTCCTTTACCGACGTAACTTATCAAC
TTTTCTGTTCTCCTATTGTGCAGTTTGTGAAGGAATTGGCTGATATCAAATCACAGCTAG
CAGCAACACATGCAACTGCAGAGGCAAGTGCTTTGTCAGCTGAATCAGCACATTCTCATT
GTAGAGTGCTTTCGAAACAATTGCATGAGAGGACCGGTTCTTTGAAAGAGCATGAGGACC
AAGTAACTAGACTTGGTGAGCAGCTAGAGAATCTAAGAAAGGAGCTGCGAGTTAGAGAAT
CTTCACAGAAGCAGCTAAGAGATGAGCTTTTGAAAGTTGAAGGTGACATTATGCGGGCTG
TATCAGTGGTCAAGACCAAGGAGAACTCTGAGGTGCGGAACATGCTAAATGAAGATACTC
CAAAGAATTCTGAAAGAATCAACAAACTTTTGACGGCTAAAGATGATGAAATTGCAAGAC
TGAGAGATGAACTGAAGATTATA

Last Updated on 06.12.2018 16:11