Sequences from confirmation amplicon


Confirmation sequences for line 221D07 and hit At5g03840 are shown.
Line ID 221D07
Locus At5g03840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1025280
Primers used for PCR MR68 - 8474, show primer details
   
Sequence Name 11-K018755-022-221-D07C-MR68
GenBank Accession LN490906 [GenBank]
Sequencing primer gene specific primer, MR68: 5'-GCAAATTTACGAGTGTTGAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K018755-022-221-D07C-MR68
GGATATTACGAAAGATAACACGTCTTTGCTTCTGCCTGAACAGAACAAACACAAACCTAT
GTATCCCTATGCTTGGCCTTGGCAATTCATAGCTCACCACCTCTTTGCCTATATTACAAT
GCAAATATTTTTATTTCTATATAACATGATATATGTATAAGTATTTAAATTACAAGATTC
ATGAAGAGGCCTTACCAAACGTAGCATCTGTTGTGCCGGGAATGTTTGTAACGATCCTAT
TGAAACATTCAACAATACTAATATTATTACTTTACAGAAAAAGTTCTGAGAACTCATGGC
GATGGAGATCTTTGACCAACTCAAATGTATATAACTTACACCCAAAAAAAACTTTATTAG
GGTTATAACGAAATGATTTAAATGCAATGTATATGGAATATGGGCCCAGATGATCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATT
   
Sequence Name 12-K018755-022-221-D07C-8409
GenBank Accession LN491366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K018755-022-221-D07C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATCATCTGGGC
CCATATTCCATATACATTGCATTTAAATCATTTCGTTATAACCCTAATAAAGTTTTTTTT
GGGTGTAAGTTATATACATTTGAGTTGGTCAAAGATCTCCATCGCCATGAGTTCTCAGAA
CTTTTTCTGTAAAGTAATAATATTAGTATTGTTGAATGTTTCAATAGGATCGTTACAAAC
ATTCCCGGCACAACAGATGCTACGTTTGGTAAGGCCTCTTCATGAATCTTGTAATTTAAA
TACTTATACATATATCATGTTATATAGAAATAAAAATATTTGCATTGTAATATAGGCAAA
GAGGTGGTGAGCTATGAATTGCCAAGGCCAAGCATAGGGATACATAGGTTTGTGTTTGTT
CTGTTCAGGCAGAAGCAAAGACGTGTTATCTTTCCTAATATCCCTTCGAGAGATCACTTC
AACACTCGTNAATTT

Last Updated on 06.12.2018 16:11