Sequences from confirmation amplicon


Confirmation sequences for line 222A08 and hit At5g43745 are shown.
Line ID 222A08
Locus At5g43745 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17571954
Primers used for PCR 06kn - 8474, show primer details
   
Sequence Name 75-K113669-0022-222-A08C-06kn
GenBank Accession LN513278 [GenBank]
Sequencing primer gene specific primer, 06kn: 5'-TGACATGCTAGCTATTAAGCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113669-0022-222-A08C-06kn
ATTAATTAATGATCATCTTGGTTTTGCATATATAGTTTCAGTTCTGATATAATGTCCCTA
CCAGTGCAGTTGTAGTCTTAACCTGACGAAATCATTTGAGAGGGCTGCCGCCTCTATGGC
GCGTGCCATTATTATACTACCAACGAAAGGTGATCGGTAAGAATATTTTCAAGTCTCTGA
AAGTATCTGTATTTTGAGGAAGAAGATTATGAAAACTGAGAAGTTCCGTATAGATATTGG
AGTAGTAGATTCTGTTAGTGTTGTAGCTCTTTCATGTTAACTGTTTCCTGGTAGTAAGAT
GCATATGGATGCGTTTTAGATAACTCCTTGATAACTTCTAATTACGCTTAGTTTACTTTG
GTTCTCAGTGAATCATACTCATTTAAGCCATGTGAGGTTTGAGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGATTATA
   
Sequence Name 44-K113666-0022-222-A08C-8409
GenBank Accession LN505251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113666-0022-222-A08C-8409
CTTAATGAGTATGATTCACTGAGAACCAAAGTAAACTAAGCGTAATTAGAAGTTATCAAG
GAGTTATCTAAAACGCATCCATATGCATCTTACTACCAGGAAACAGTTAACATGAAAGAG
CTACAACACTAACAGAATCTACTACTCCAATATCTATACGGAACTTCTCAGTTTTCATAA
TCTTCTTCCTCAAAATACAGATACTTTCAGAGACTTGAAAATATTCTTACCGATCACCTT
TCGTTGGTAGTATAATAATGGCACGCGCCATAGAGGCGGCAGCCCTCTCAAATGATTTCG
TCAGGTTAAGACTACAACTGCACTGGTAGGGACATTATATCAGAACTGAAACTATATATG
CAAAACCAAGATGATCATTAATTAATATGGCATGAACCTAAGCAGCATTTCAGGTTCACT
TTCGCTTAATACTNGNCATGTC

Last Updated on 06.12.2018 16:11