Sequences from confirmation amplicon


Confirmation sequences for line 222C01 and hit At4g18100 are shown.
Line ID 222C01
Locus At4g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10036216
Primers used for PCR 06wm - 8474, show primer details
   
Sequence Name 33-K118761-0022-222-C01C-06wm
GenBank Accession LN501192 [GenBank]
Sequencing primer gene specific primer, 06wm: 5'-AGGCCGTGAAAGAAATCTTATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118761-0022-222-C01C-06wm
ATATCGAAACCCTANTGTACGCTTATAAAGACATTTTAGGTTTGTTCAGCTGTTTTACGG
AGTCGCTTTTGAGATATCGATCTTACGAAGAAGCAAATATGGCGGTCCCATTGCTTACCA
AGAAGGTTGTGAAGAAGAGGTCTGCTAAGTTCATCAGACCCCAGAGTGACCGTAGAATCA
CCGTCAAGGTAAACCCTTTTTCTCATTTGATTTCACAGAGTTTTAGGATTTTAGTAGATC
AATCTCTGGGTTTTGTTCCTTTAAGTTTTTATTGGTAGCTGTATCTTAAAGTTTGTAGCT
TTAGGATGCAATATGGTGGTTGAATTAAACATCTGACATTAGTCTCTATCTATGAAGAAA
AAGAAATATATTGACATCTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K118760-0022-222-C01C-8409
GenBank Accession LN489943 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118760-0022-222-C01C-8409
GCCATTTCATTGAATATATCAGATGTCAATATATTTCTTTTTCTTCATAGATAGAGACTA
ATGTCAGATGTTTAATTCAACCACCATATTGCATCCTAAAGCTACAAACTTTAAGATACA
GCTACCAATAAAAACTTAAAGGAACAAAACCCAGAGATTGATCTACTAAAATCCTAAAAC
TCTGTGAAATCAAATGAGAAAAAGGGTTTACCTTGACGGTGATTCTACGGTCACTCTGGG
GTCTGATGAACTTAGCAGACCTCTTCTTCACAACCTTCTTGGTAAGCAATGGGACCGCCA
TATTTGCTTCTTCGTAAGATCGATATCTCAAAAGCGACTCCGTAAAACAGCTGAACAAAC
CTAAAATGTCTTTTATAAGCGTACACTAGGGTTTCTGATATTACATTACTAAATGGGCTC
ACGTTGTGGCCCAATAAGATTTCTTTCACGGCC

Last Updated on 06.12.2018 16:11