Sequences from confirmation amplicon


Confirmation sequences for line 222C12 and hit At4g33180 are shown.
Line ID 222C12
Locus At4g33180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16000893
Primers used for PCR 08eb - 8474, show primer details
   
Sequence Name 23-K110307-0022-222-C12C-08eb
GenBank Accession LN496601 [GenBank]
Sequencing primer gene specific primer, 08eb: 5'-TTGGATCTTTGCAATAAACTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K110307-0022-222-C12C-08eb
TGCCGGAGCTGCATCACCACTTTCTCACTTTTTCCGGCCACATTTTGGCCATGTGGTACG
CAACAAACCCCCCGTAGCTTGTTCCAGCCACATTATACTTCCCTATTCCTATTTTCGCCA
TTAGCTTTGCCATACATTCCGCCTAATTGTTTTATATTACGTATATGTTTTAATACTAAA
AAGAGTTCAAAACATATTTTATTTAAACACGACTATCGCTTATTTCACTTTTGAGATACT
TTCTAGAGAGCATGAAGGATTGATCATAATATACTGATCACAGTTTTGAGATTCAGATAT
TAAAAATATGTAGTAGCATTGTAGCAAGGAAATCAAATGATTATATATTTAACAAAAAAG
GAACATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 07-K110306-0022-222-C12C-oriL
GenBank Accession LN489322 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K110306-0022-222-C12C-oriL
ATTGAATATATGTTCCTTTTTTGTAAATATATAATCATTTGATTTCCTTGCTACAATGCT
ACTACATATTTTTAATATCTGAATCTCAAAACTGTGATCAGTATATTATGATCAATCCTT
CATGCTCTCTAGAAAGTATCTCAAAAGTGAAATAAGCGATAGTCGTGTTTAAATAAAATA
TGTTTTGAACTCTTTTTAGTATTAAAACATATACGTAATATAAAACAATTAGGCGGAATG
TATGGCAAAGCTAATGGCGAAAATAGGAATAGGGAAGTATAATGTGGCTGGAACAAGCTA
CGGGGGGTTTGTTGCGTACCACATGGCCAAAATGTGGCCGGAAAAAGTGGAGAAAGTGGT
GATTGCAAGCTCCGGCATCAACATGCGAAAGTGTGACGGTGAAAGTTTATTGCAAAGATC
CA
Primers used for PCR 05do - 8474, show primer details
   
Sequence Name 19-K110307-0022-222-C12C-05do
GenBank Accession LN494804 [GenBank]
Sequencing primer gene specific primer, 05do: 5'-AGAGCATCAGGAAACATACGAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K110307-0022-222-C12C-05do
CAAGCCATAAGTGTGCGAACTCAGTTGCAGTGGATGGTAACATAACCTTCTCGATGCACT
CACAATTGGATCTTTGCAATAAACTTTCACCGTCACACTTTCGCATGTTGATGCCGGAGC
TTGCAATCACCACTTTCTCCACTTTTTCCGGCCACATTTTGGCCATGTGGTACGCAACAA
ACCCCCCGTAGCTTGTTCCAGCCACATTATACTTCCCTATTCCTATTTTCGCCATTAGCT
TTGCCATACATTCCGCCTAATTGTTTTATATTACGTATATGTTTTAATACTAAAAAGAGT
TCAAAACATATTTTATTTAAACACGACTATCGCTTATTTCACTTTTGAGATACTTTCTAG
AGAGCATGAAGGATTGATCATAATATACTGATCACAGTTTTGAGATTCAGATATTAAAAA
TATGTAGTAGCATTGTAGCAAGGAAATCAAATGATTATATATTTAACAAAAAAGGAACAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 03-K110306-0022-222-C12C-8409
GenBank Accession LN487534 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K110306-0022-222-C12C-8409
ATTGAATATATGTTCCTTTTTTGTAAATATATAATCATTTGATTTCCTTGCTACAATGCT
ACTACATATTTTTAATATCTGAATCTCAAAACTGTGATCAGTATATTATGATCAATCCTT
CATGCTCTCTAGAAAGTATCTCAAAAGTGAAATAAGCGATAGTCGTGTTTAAATAAAATA
TGTTTTGAACTCTTTTTAGTATTAAAACATATACGTAATATAAAACAATTAGGCGGAATG
TATGGCAAAGCTAATGGCGAAAATAGGAATAGGGAAGTATAATGTGGCTGGAACAAGCTA
CGGGGGGTTTGTTGCGTACCACATGGCCAAAATGTGGCCGGAAAAAGTGGAGAAAGTGGT
GATTGCAAGCTCCGGCATCAACATGCGAAAGTGTGACGGTGAAAGTTTATTGCAAAGATC
CAATTGTGAGTGCATCGAGAAGGTTATGTTACCATCCACTGCAACTGAGTTTCGCACACT
TATGGCTTTGGCATCTTCATGGCGGTTAGTTCGTATGTTTCCTGATGCTC

Last Updated on 06.12.2018 16:11