Sequences from confirmation amplicon


Confirmation sequences for line 222D01 and hit At1g17100 are shown.
Line ID 222D01
Locus At1g17100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5844995
Primers used for PCR KA26 - 8474, show primer details
   
Sequence Name 66-K106189-0022-222-D01C-KA26
GenBank Accession LN511615 [GenBank]
Sequencing primer gene specific primer, KA26: 5'-GAAGGGTGAGTTATACTGAGCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106189-0022-222-D01C-KA26
GTCTTCTTTGCTTTCGCTATGCATTAGCCCAAGCCGTACCTTTGAGGCTCGAGTCTAGCG
CCGCCGCTTGTTCTCCTATGCTATCATCAGACACGAATCCACTGAATTGTCTCACGGCCA
CGTACCTAGAGTTCCATTTCTGAATGTGGAGATTCTCCGACGGAGCTGGATCAGGCTGGT
TCTTTTTAGGAACGTAGAATGATACAGTGAAGGAAGATTCACAGAATGGACCGTCGCTTG
GTGAGACTTGAGAGATAACCGGAGCAGTCATTTCTATCTTCTGATGATACTCGTTCTTCC
CCTGAATGTATGCAAACAATCTGATACCAAAATTTTCAATCTTTATCCGAACAAAAGCCT
CATTTTAAAAGATAAAACTGGAAATTTGCACTTACTGGAAGAAAGCGGTTCTGGTAGCAT
CAACGAGAGAGATATCTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCC
   
Sequence Name 58-K106190-0022-222-D01C-8409
GenBank Accession LN509712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K106190-0022-222-D01C-8409
ATATCTCTCTCGTTGATGCTACCAGAACCGCTTTCTTCCGTAAGTGCAAATTTCCAGTTT
TATCTTTTAAAATGAGGCTTTTGTTCGGATAAAGATTGAAAATTTTGGTATCAGATTGTT
TGCATACATTCAGGGGAAGAACGAGTATCATCAGAAGATAGAAATGACTGCTCCGGTTAT
CTCTCAAGTCTCACCAAGCGACGGTCCATTCTGTGAATCTTCCTTCACTGTATCATTCTA
CGTTCCTAAAAAGAACCAGCCTGATCCAGCTCCGTCGGAGAATCTCCACATTCAGAAATG
GAACTCTAGGTACGTGGCCGTGAGACAATTCAGTGGATTCGTGTCTGATGATAGCATAGG
AGAACAAGCGGCGGCGCTAGACTCGAGCCTCAAAGGTACGGCTTGGGCTAATGCAATAGC
GAAAAGCAAAGAAGACGGTGGTGTTGGGTCGGATTCAGCTTACACGGTCGCTCAGTATAC
TNCACCCTT
Primers used for PCR 32au - 3144, show primer details
   
Sequence Name 04-K106829-0022-222-D01C-3144
GenBank Accession LN487938 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106829-0022-222-D01C-3144
CCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTCTGGAAGACGGTGTTGTTGTACCGGCGA
ATTTCGTATCCATTTCCGGAGTGAACCAGCTCGTAGCTCGGGCATTCGATTCTGTTACAC
GACGGCGGGAACTTCCCAATTTGGGCGACTCCGGATTCGGCATCGGGACCTAATAGGTCT
GATCCACCGGAGAGGAGGCTAAGGAGGAAGGAGAGCTTAAAGAAGCCTAAACCGGTGGCC
ATGGCGGTTTTATCTAGGTAGCTTACTTCTTTCTTTGTTTACGCGGCCTTTTACCGGTAA
TATAGATTATTTGTAACATGTGAATGTGTGGT
   
Sequence Name 09-K106830-0022-222-D01C-32au
GenBank Accession LN490197 [GenBank]
Sequencing primer gene specific primer, 32au: 5'-ACCACACATTCACACGTAACAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106830-0022-222-D01C-32au
ACGGTAGGCGCGTAACAAGAAGAAGTAGCTACCTAGATAAACCGCCATGGCCACCGGTTT
AGGCTTCTTTAAGCTCTCCTTCCTCCTTAGCCTCCTCTCCGGTGGATCAGACCTATTAGG
TCCCGATGCCGAATCCGGAGTCGCCCAAATTGGGAAGTTCCCGCCGTCGTGTAACAGAAT
CGAATGCCCGAGCTACGAGCTGGTTCACTCCGGAAATGGATACGAAATTCGCCGGTACAA
CAACACCGTCTTCCAGAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTC
CTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGAT
TGTGCGTCATCCCTTACGTCAGTGGAGATATAACATCAATCCACAC

Last Updated on 06.12.2018 16:11