Sequences from confirmation amplicon


Confirmation sequences for line 222E11 and hit At1g80830 are shown.
Line ID 222E11
Locus At1g80830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30374646
Primers used for PCR 36ra - 8474, show primer details
   
Sequence Name 40-K115806-0022-222-E11C-36ra
GenBank Accession LN503822 [GenBank]
Sequencing primer gene specific primer, 36ra: 5'-ATGAAGTGCGTTCATACTCTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115806-0022-222-E11C-36ra
GCATTTTTACAAATCATGATTTCTTTCTTTGTAATAACAGTTACTTTGGATCATATTGGT
GGCCTCATGTGCGGCTTTGGTGATTCAATCTCTGGCTGCTAATCTTGGTGTTGTCACAGG
TTACCACAAAAATCCACTCTTTTCTTTTAGTTTTATGATCCAAGTTTACTAAATTCCGCT
ACAGGAAAACATTTGGCTGAGCAATGTAGAGCCGAGTACTCCAAAGTTCCAAACTTTATG
TTATGGGTCGTTGCTGAAATTGCAGTAGTTGCTTGTGACATACCGGAAGGTATCACACTT
GTTTACTCTTTACAGTGTGATTCAATACTAACCTCTCTCTGTTGATACTAATTGCCAAAA
TCGGTTTTCTTATATATATATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATCTCCGCAC
   
Sequence Name 19-K115805-0022-222-E11C-8409
GenBank Accession LN494902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115805-0022-222-E11C-8409
TTGAATATATATATATATATAAGAAAACCGATTTTGGCAATTAGTATCAACAGAGAGAGG
TTAGTATTGAATCACACTGTAAAGAGTAAACAAGTGTGATACCTTCCGGTATGTCACAAG
CAACTACTGCAATTTCAGCAACGACCCATAACATAAAGTTTGGAACTTTGGAGTACTCGG
CTCTACATTGCTCAGCCAAATGTTTTCCTGTAGCGGAATTTAGTAAACTTGGATCATAAA
ACTAAAAGAAAAGAGTGGATTTTTGTGGTAACCTGTGACAACACCAAGATTAGCAGCCAG
AGATTGAATCACCAAAGCCGCACATGAGGCCACCAATATGATCCAAAGTAACTGTTATTA
CAAAGAAAGAAATCATGATTTTGTAAAAAATGCAAACTAGTATAAGATAAAAGCGAGGGA
AGGAGAGTATGAACGCACTTCA

Last Updated on 06.12.2018 16:11