Sequences from confirmation amplicon


Confirmation sequences for line 223B04 and hit At4g23420 are shown.
Line ID 223B04
Locus At4g23420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12227663
Primers used for PCR 17pv - 8474, show primer details
   
Sequence Name 40-K115234-0022-223-B04C-17pv
GenBank Accession LN503811 [GenBank]
Sequencing primer gene specific primer, 17pv: 5'-CCAGAATTGAAGATAACCATGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115234-0022-223-B04C-17pv
TGTAGTTACCTTCAGTTGCTTTGCAAGCTCGTTGGCGTGCAATACATTGCAGAGTTTAGA
TTGTCCATAAGCACGTATACTACTGTAACTGCATGTGGAGACCAAATCAAGGAAGAATGT
TGGCTTTAGAAGAAAAGATGGGATCGGAGAATTATACTATTTGGTTCACCATTAGTACCT
TGACTCGTCGTTGATCTTATCAAAGCGGACTCCTTCTGGGTACGAGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAACGTTATT
   
Sequence Name 16-K115233-0022-223-B04C-8409
GenBank Accession LN493552 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115233-0022-223-B04C-8409
ATTACTCGTACCCAGAAGGAGTCCGCTTTGATAAGATCAACGACGAGTCAAGGTACTAAT
GGTGAACCAAATAGTATAATTCTCCGATCCCATCTTTTCTTCTAAAGCCAACATTCTTCC
TTGATTTGGTCTCCACATGCAGTTACAGTAGTATACGTGCTTATGGACAATCTAAACTCT
GCAATGTATTGCACGCCAACGAGCTTGCAAAGCAACTGAAGGTAAACTACACTAAACAAA
GCAAAGATATAGCAAATCAAGAATCTATGTTTCCATGGTTATCTTCAATTCTGG

Last Updated on 06.12.2018 16:11