Sequences from confirmation amplicon


Confirmation sequences for line 223D10 and hit At1g12850 are shown.
Line ID 223D10
Locus At1g12850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4380916
Primers used for PCR BJ27 - 8474, show primer details
   
Sequence Name 10-K104372-0022-223-D10C-BJ27
GenBank Accession LN490637 [GenBank]
Sequencing primer gene specific primer, BJ27: 5'-AGCGAAGGTGAAGAAGTAGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104372-0022-223-D10C-BJ27
TACAGAAGATCGAGCTGGATAATCTCTTGGTCAAGAAAGTTCTCGAGCAAGAGCCTGAGA
TGCTTCCTTGTCACGCCTCTGCTTCTCCACTCTCTCCTCAGCTCTCTTCTCTTGGAACTC
CTCGAATCGGACCTTCCATTAAAGTCTGGGATCCTTACAACGTCCTCGCGCCACCTCCTC
CTTCTTCTCCGCCTCTTTTCTCTCGTATCTCCTCCGCCGCGGAACACGATCGCTCCGCCG
TGACGGAAGTCTATTTCATCAGCCACGGCGAGTGTGATCTCAATCTCAGGCCTGATCTGA
TTGGAGGTAGATGCCACGTGGCTACTCTCACACCCAACGGGAAACGTCAGGCGAGAGCTC
TAGCCGTATTTTTAAACTCTGAAGGTGTTCGATTCACCTCCGTCTTCTCTTCGCCTCTGG
ATCGAGCTAGATCCATGGCTGTTTCTGTTTGCCAGGTACAGTCTCTACTCTTCGATTTCT
CAATTCTAGATCTAAGGCTGTGAAATTGGAATCCAATTCTTCAAAATTAGGTATTAGTAT
AGTAATGATTTAGGTAGAGTAATGGTACATGGATCATACGATTTACAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 26-K104373-0022-223-D10C-8409
GenBank Accession LN497907 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104373-0022-223-D10C-8409
CATTTACAATTGTAAATCGTATGATCCATGTACCATTACTCTACCTAAATCATTACTATA
CTAATACCTAATTTTGAAGAATTGGATTCCAATTTCACAGCCTTAGATCTAGAATTGAGA
AATCGAAGAGTAGAGACTGTACCTGGCAAACAGAAACAGCCATGGATCTAGCTCGATCCA
GAGGCGAAGAGAAGACGGAGGTGAATCGAACACCTTCAGAGTTTAAAAATACGGCTAGAG
CTCTCGCCTGACGTTTCCCGTTGGGTGTGAGAGTAGCCACGTGGCATCTACCTCCAATCA
GATCAGGCCTGAGATTGAGATCACACTCGCCGTGGCTGATGAAATAGACTTCCGTCACGG
CGGAGCGATCGTGTTCCGCGGCGGAGGAGATACGAGAGAAAAGAGGCGGAGAAGAAGGAG
GAGGTGGCGCGAGGACGTTGTAAGGATCCCAGACTTTAATGGAAGGTCCGATTCGAGGAG
TTCCAAGAGAAGAGAGCTGAGGAGAGAGTGGAGAAGCAGAGGCGTGACAAGGAAGCATCT
CAGGCTCTTGCTCGAGAACTTTCTTGACCAAGAGATTATCCAGCTCGATTCTTCTGTTAT
TCAATTCGCTCTCTGCTTCATCTTCTTCTTCTTCTACT

Last Updated on 06.12.2018 16:11