Sequences from confirmation amplicon


Confirmation sequences for line 223H09 and hit At1g74045 are shown.
Line ID 223H09
Locus At1g74045 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27846258
Primers used for PCR EP10 - 8474, show primer details
   
Sequence Name 56-K102176-0022-223-H09C-8409
GenBank Accession LN509056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102176-0022-223-H09C-8409
TTACATTGAATAATCATTGTATCTTATTATTCAATCAAATGTTCCATTTACAACACCACT
TTTGGCATAGCTAAGAATCCCGCCAAGGACAACCGTACCGTGCCACAGTTACTTGGTAGG
TTGGTCTCTAAGGAGAAGTTCGAGAAAGTAACCTATTGTATCATTCACAAACATGACTGT
AATTACAATGCCAGCAAAAACAGCAATGTTTGGGTAAGTTTTGAATCTTTTTACTATTAA
TAAGAGACATGTCTATATGAAAATGTGCATGCTTATATTTAGGATATATGTGCAGAAGTA
TTGCTGTGCACAACCGGTGGGATGTGGGACCATAACGATGTTCGACAAGCCAGGCGAGTG
GAGTTGGAAACATCAGTATGAACGGAACCAAGTTCCAGAAGAGTGTTCTTATGAGTATTG
TTTAGATTGCAGAGGCTGCCAACTAAGCATCTTAAAAGCCATTGTTCATCAATGGAAATA
TCTCTCGATGTTGCCTTACC
   
Sequence Name 56-K102180-0022-223-H09C-EP10
GenBank Accession LN509057 [GenBank]
Sequencing primer gene specific primer, EP10: 5'-AGGGTAAGCAAACATCGAGAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102180-0022-223-H09C-EP10
TGGCTTTAGAGCTAGTTGGCAGCCTCTGCAATCTAAACAATACTCATAAGAACACTCTTC
TGGAACTTGGTTCCGTTCATACTGATGTTTCCAACTCCACTCGCCTGGCTTGTCGAACAT
CGTTATGGTCCCACATCCCACCGGTTGTGCACAGCAATACTTCTGCACATATATCCTAAA
TATAAGCATGCACATTTTCATATAGACATGTCTCTTATTAATAGTAAAAAGATTCAAAAC
TTACCCAAACATTGCTGTTTTTGCTGGCATTGTAATTACAGTCATGTTTGTGAATGATAC
AATAGGTTACTTTCTCGAACTTCTCCTTAGAGACCAACCTACCAAGTAACTGTGGCACGG
TACGGTTGTCCTTGAGGGGATTCTTAGCTATGCCAAAAGTGGTGTTGTAAATGGAACATT
TGATTGAATAATAAGATACAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATAAA

Last Updated on 06.12.2018 16:11