Sequences from confirmation amplicon


Confirmation sequences for line 224B12 and hit FCAALL are shown.
Line ID 224B12
Locus FCAALL [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9422530
Primers used for PCR PE01 - 8474, show primer details
   
Sequence Name 27-K027845-022-224-B12C-PE01
GenBank Accession LN498237 [GenBank]
Sequencing primer gene specific primer, PE01: 5'-CTAGTGGGTTCATTCCAATTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K027845-022-224-B12C-PE1
GTTATTTATGTCAATATTTAACATTTTCGCATCAAAATGGAGTATATAGGACATACTTTA
ACGGTTTAACCCCTGCTAAAATGACAAGAATTCAGATATTCAATAACGTGATACAATATA
TAAAAATATATAGATTATATATATAAACGTAAATACTACTATTATTACCTGAGGCATTTT
TTAATGAGTTAACTCTTAAGACGAATTTTATTTTATTGCTAAATTAGTTTTATTTGTCGA
AATGTGAATTAAATTAATTAATAAAAAAAGATATTTACAAAAGTGTTTGGTCAAGAAAAG
AAACAGAGCAATAATATCAGAACCAAAACCAGAGAAACTTATGAAACAGAGCACACAAAG
CTAACGAACTAGGAGTTGCGTCTCGACTGAAACCATAGCTGAAGTAGTCCCTGGAGGCTG
GAGCTTCCGATTTTCGAAATAGTGTAGAGGCGTATACTGCTAAATTAATAATGAGATTTA
AGATGTATTAATATTAACCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTC
   
Sequence Name 26-K027845-022-224-B12C-8409
GenBank Accession LN497773 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K027845-022-224-B12C-8409
CATTTACAGGTTAATATTAATACATCTTAAATCTCATTATTAATTTAGCAGTATACGCCT
CTACACTATTTCGAAAATCGGAAGCTCCAGCCTCCAGGGACTACTTCAGCTATGGTTTCA
GTCGAGACGCAACTCCTAGTTCGTTAGCTTTGTGTGCTCTGTTTCATAAGTTTCTCTGGT
TTTGGTTCTGATATTATTGCTCTGTTTCTTTTCTTGACCAAACACTTTTGTAAATATCTT
TTTTTATTAATTAATTTAATTCACATTTCGACAAATAAAACTAATTTAGCAATAAAATAA
AATTCGTCTTAAGAGTTAACTCATTAAAAAATGCCTCAGGTAATAATAGTAGTATTTACG
TTTATATATATAATCTATATATTTTTATATATTGTATCACGTTATTGAATATCTGAATTC
TTGTCATTTTAGCAGGGGTTAAACCGTTAAAGTATGTCCTATATACTCCATTTTGATGCG
AAAATGTTAAATATTGACATAAAATAACATAGACTTATTAATAAAAGTGAAAGAATTG
Primers used for PCR PE03 - 8474, show primer details
   
Sequence Name 03-K027846-022-224-B12C-PE03
GenBank Accession LN487343 [GenBank]
Sequencing primer gene specific primer, PE03: 5'-TAGTGGGTTCATTCCAATTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K027846-022-224-B12C-PE3
ACTATGTTATTTTATGTCATATTAACATTTTCGCATCAAAATGGAGTATATAGGACATAC
TTTAACGGTTTAACCCCTGCTAAAATGACAAGAATTCAGATATTCAATAACGTGATACAA
TATATAAAAATATATAGATTATATATATAAACGTAAATACTACTATTATTACCTGAGGCA
TTTTTTAATGAGTTAACTCTTAAGACGAATTTTATTTTATTGCTAAATTAGTTTTATTTG
TCGAAATGTGAATTAAATTAATTAATAAAAAAAGATATTTACAAAAGTGTTTGGTCAAGA
AAAGAAACAGAGCAATAATATCAGAACCAAAACCAGAGAAACTTATGAAACAGAGCACAC
AAAGCTAACGAACTAGGAGTTGCGTCTCGACTGAAACCATAGCTGAAGTAGTCCCTGGAG
GCTGGAGCTTCCGATTTTCGAAATAGTGTAGAGGCGTATACTGCTAAATTAATAATGAGA
TTTAAGATGTATTAATATTAACCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 02-K027846-022-224-B12C-8409
GenBank Accession LN486894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K027846-022-224-B12C-8409
AGCCATTTACAAGGTTAATATTAATACATCTTAAATCTCATTATTAATTTAGCAGTATAC
GCCTCTACACTATTTCGAAAATCGGAAGCTCCAGCCTCCAGGGACTACTTCAGCTATGGT
TTCAGTCGAGACGCAACTCCTAGTTCGTTAGCTTTGTGTGCTCTGTTTCATAAGTTTCTC
TGGTTTTGGTTCTGATATTATTGCTCTGTTTCTTTTCTTGACCAAACACTTTTGTAAATA
TCTTTTTTTATTAATTAATTTAATTCACATTTCGACAAATAAAACTAATTTAGCAATAAA
ATAAAATTCGTCTTAAGAGTTAACTCATTAAAAAATGCCTCAGGTAATAATAGTAGTATT
TACGTTTATATATATAATCTATATATTTTTATATATTGTATCACGTTATTGAATATCTGA
ATTCTTGTCATTTTAGCAGGGGTTAAACCGTTAAAGTATGTCCTATATACTCCATTTTGA
TGCGAAAATGTTAAATATTGACATAAAATAACATAGACTTATTAATAAAAGTGAAAGAAT
TGG

Last Updated on 06.12.2018 16:11