Sequences from confirmation amplicon


Confirmation sequences for line 224F03 and hit At1g34430 are shown.
Line ID 224F03
Locus At1g34430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12589952
Primers used for PCR BB24 - 8474, show primer details
   
Sequence Name 35-K103847-0022-224-F03C-BB24
GenBank Accession LN501806 [GenBank]
Sequencing primer gene specific primer, BB24: 5'-CAACCTTCTTCTCAACACTCACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103847-0022-224-F03C-BB24
GCCGGAGGAGCCTTAGAATCACCACCACCACCACCACCAGAAGCTTTAGCTTTAGCATCA
GCAATCTCATCTTCAGTTTCAGCTAACAAAGCAATAGCAGAACCAACAGGAGCAACACCA
CCTTCTTCAACCATGATTGCAGCTAGATAACCATCATAGAAAGTCTCAACATCCATATCA
GCTTTATCAGATTCAACAACAACAACACTCTCTCCTTTGTTGAGTTTATCGCCTTCTGAT
TTAACCCAAGAGACGATTTTGCCTTCGGTCATCGTGGAACTTAGTGCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACAATTATA
   
Sequence Name 51-K103848-0022-224-F03C-8409
GenBank Accession LN507482 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103848-0022-224-F03C-8409
CGGACATGAGCCTTTACATTGAATATATCCTGCACTAAGTTCCACGATGACCGAAGGCAA
AATCGTCTCTTGGGTTAAATCAGAAGGCGATAAACTCAACAAAGGAGAGAGTGTTGTTGT
TGTTGAATCTGATAAAGCTGATATGGATGTTGAGACTTTCTATGATGGTTATCTAGCTGC
AATCATGGTTGAAGAAGGTGGTGTTGCTCCTGTTGGTTCTGCTATTGCTTTGTTAGCTGA
AACTGAAGATGAGATTGCTGATGCTAAAGCTAAAGCTTCTGGTGGTGGTGGTGGTGGTGA
TTCTAAGGCTCCTCCGGCTTCTCCTCCCACGGCTGCAGTTGAAGCACCTGTGAGTGTTGA
GAAGAAGGTTG

Last Updated on 06.12.2018 16:11