Sequences from confirmation amplicon


Confirmation sequences for line 224H12 and hit At2g39750 are shown.
Line ID 224H12
Locus At2g39750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16579709
Primers used for PCR 02ek - 8474, show primer details
   
Sequence Name 24-K110713-0022-224-H12C-02ek
GenBank Accession LN497040 [GenBank]
Sequencing primer gene specific primer, 02ek: 5'-TACACAAATCTGAAACCTTGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K110713-0022-224-H12C-02ek
ACGTCCCCTTATGGCCTGCCCGTCTGCATACTCCACCTGATAGGCTGCAGACGATAAAAT
TTGATTCCTACATTGCTAGGAAAGAGCTATTCAAGGCAGAATCAAAATATTGGAATGAAA
TAATTGGGGGCTATGTTCGCGCCTTAAAATGGAAAAAGATGAAACTAAGAAATGTTTTGG
ACATGAGAGCAGGCTTTGGCGGGTATGAAATAATATTTCTTTGTTAGGTATTATACAGGT
ATCTTAGCTTATCTCTCTTCATTTATTCCTTTGGCTTTGGCAGATTTGAAGAAAAATCCC
ATCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGATTA
   
Sequence Name 08-K110711-0022-224-H12C-8409
GenBank Accession LN489782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K110711-0022-224-H12C-8409
TTACATTGAATATATCCTGGGATGGGATTTTTCTTCAAATCTGCCAAAGCCAAAGGAATA
AATGAAGAGAGATAAGCTAAGATACCTGTATAATACCTAACAAAGAAATATTATTTCATA
CCCGCCAAAGCCTGCTCTCATGTCCAAAACATTTCTTAGTTTCATCTTTTTCCATTTTAA
GGCGCGAACATAGCCCCCAATTATTTCATTCCAATATTTTGATTCTGCCTTGAATAGCTC
TTTCCTAGCAATGTAGGAATCAAATTTTATCGTCTGCAGCCTATCAGGTGGAGTATGCAG
ACGGGCAGGCCATAAGGGGACGTTTCCTCCGTACCCTTTCTCAGGTATGCGACTGATGCA
AGGTTTCAGATTTGTGT

Last Updated on 06.12.2018 16:11