Sequences from confirmation amplicon


Confirmation sequences for line 225A03 and hit At1g13620 are shown.
Line ID 225A03
Locus At1g13620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4668439
Primers used for PCR 14sa - 8474, show primer details
   
Sequence Name 49-K116229-0022-225-A03C-14sa
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 14sa: 5'-AGTTGGATTGGCTCAGTTTCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116229-0022-225-A03C-14sa
AAAATTAAGAACATTGACTCAATATTCACTTTCAAGTCTTTTCTTGTGTTTTGTTTGATT
CAATTCGTAAATTGGCTTTCATTGTTCCGGGAAAATTCTACATTGGATTCAGTCAATTGA
GTTATTGAATTGGTCAATATTGGAGAAAAAAGTAAAGAAGTAATTTACTCAATTTTCTTT
TTCAATTTCAAAAA
   
Sequence Name 09-K116228-0022-225-A03C-8409
GenBank Accession LN490344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116228-0022-225-A03C-8409
CAAATTCACGCAAGAAATGATGAAGAATTAGAAGAAAGGTTAACTAGTTGTTTATATAGG
AAGATCTTAAGAAAGAAAAACCCCCAAGAGAATTTCAAATCTAATCTATTTTTATCTTAC
CATAAGCCATGTGTCTTTATTGGGAACCTGTCATGCATAGACATTGGGACATGCGTTAAA
ATCCCCGAACAATGTAAGAAACTGTGAATTACAACATGAGATCCAAGTTCACACCCATAA
AGAAACTGA

Last Updated on 06.12.2018 16:11