Sequences from confirmation amplicon


Confirmation sequences for line 225A04 and hit At3g25950 are shown.
Line ID 225A04
Locus At3g25950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9496883
Primers used for PCR 58we - 8474, show primer details
   
Sequence Name 75-K118585-0022-225-A04C-58we
GenBank Accession LN513305 [GenBank]
Sequencing primer gene specific primer, 58we: 5'-CCATGAGTTAGGCAACTTTTTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118585-0022-225-A04C-58we
TCATTATATAAAATTCAAACAAAATTCAAGGGCTGAAAAGAAAAGGTTTAAGCATTCAAG
TATTGACAAGTATAATTTTACATAAGATTAACATATATATAATTTTACATAAGATTAATT
TTACAAACAATTAAGAAATATTTACAATGCAAATGACAATAAGATTTAGATTCACATAAA
AGTACAATTCTGTTAATATTGCATTACACAAATACAAATCAAATAAATTTATATGAAATT
TGCTAAGAATCTATCAATTAAACAATTCACTTGTATTTCTTGCTAGAGTTATTCTCCCTA
AACCAATCAAGCCAATTACGTAAAACCCACAAAATACTAACCAAAATAGCAAACCCAATC
ACAACAAGCCACGATAACCAAGCCCACCGTGGAACCACTCCATCCGCCGCACCGCTACCG
TAAAACGTCGCCATATCATACAAAACCACCGGACCAGCCAATCCACGAACCACAGTATAA
AAAAGATAAAACGGCAATGAGCAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTTATTAT
   
Sequence Name 45-K118584-0022-225-A04C-8409
GenBank Accession LN505691 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118584-0022-225-A04C-8409
TCATTGGTAATTGCTCATTGCCGTTTTTCTTTTTTATACTGTGGTTCGTGGATTGGCTGG
TCCGGTGGTTTTGTATGATATGGCGACGTTTTACGGTAGCGGTGCGGCGGATGGAGTGGT
TCCACGGTGGGCTTGGTTATCGTGGCTTGTTGTGATTGGGTTTGCTATTTTGGTTAGTAT
TTTGTGGGTTTTACGTAATTGGCTTGATTGGTTTAGGGAGAATAACTCTAGCAAGAAATA
CAAGTGAATTGTTTAATTGATAGATTCTTAGCAAATTTCATATAAATTTATTTGATTTGT
ATTTGTGTAATGCAATATTAACAGAATTGTACTTTTATGTGAATCTAAATCTTATTGTCA
TTTGCATTGTAAATATTTCTTAATTGTTTGTAAAATTAATCTTATGTAAAATTATATATA
TGTTAATCTTATGTAAAATTATACTTGTCAATACTTGAATGCTTAAACCTTTTCTTTTCA
GCCCTTGAATTTTGTTTTGAATTTTTATATAATGATGTCAACATCCTTTAATATATGATT
TGGCTAAAAAGTTGCCTAACTCATGG

Last Updated on 06.12.2018 16:11