Sequences from confirmation amplicon


Confirmation sequences for line 225D11 and hit At4g02580 are shown.
Line ID 225D11
Locus At4g02580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1134254
Primers used for PCR CS58 - 8474, show primer details
   
Sequence Name 27-K105032-0022-225-D11C-CS58
GenBank Accession LN498379 [GenBank]
Sequencing primer gene specific primer, CS58: 5'-ATTTTCGACAATTTAGAGGACGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105032-0022-225-D11C-CS58
TTTTTTGATCTGAAAGAGGAGAACAGAGAAACCAAGGAGCTAGACTCGGTATCTCACTAT
CACCTGGCGCAGGATTGACTATAGCCTCTATGGGCCTGCTAAAATAAGCCTGACTTTAAT
TCATTATTGGTCTATTACTTTTGGTTTAGGACTGATACATACGAGTAGCTTTAAAGGCCC
ATTTTATTAATTAAAGGCCCATACATGTAATACTTGAAGCCCGTTGGAATTTGCTGATTC
TTATAATTTGCACATCTCCTCGCAGCTTTTTCATCATAGTGCAATGGCAATGGCAATTAA
GTTGGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGC
   
Sequence Name 43-K105033-0022-225-D11C-8409
GenBank Accession LN504832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K105033-0022-225-D11C-8409
CATTTACCCAACTTAATTGCCATTGCCATTGCACTATGATGAAAAAGCTGCGAGGAGATG
TGCAAATTATAAGAATCAGCAAATTCCAACGGGCTTCAAGTATTACATGTATGGGCCTTT
AATTAATAAAATGGGCCTTTAAAGCTACTCGTATGTATCAGTCCTAAACCAAAAGTAATA
GACCAATAATGAATTAAAGTCAGGCTTATTTTAGCAGGCCCATAGAGGCTATAGTCAATC
CTGCGCCAGGTGATAGTGAGATACCGAGTCTAGCTCCTTGGTTTCTCTGCTTCTCCTTCT
CTCTCAGATCAAAAAAAACCTTCGTGTGCGTCTCTCGTCCTCTAATTGATCGAAAA

Last Updated on 06.12.2018 16:11