Sequences from confirmation amplicon


Confirmation sequences for line 226C07 and hit At4g38350 are shown.
Line ID 226C07
Locus At4g38350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17966563
Primers used for PCR AH56 - 8474, show primer details
   
Sequence Name 36-K115837-0022-226-C07C-AH56
GenBank Accession LN502355 [GenBank]
Sequencing primer gene specific primer, AH56: 5'-TCTCTGCAACTAACTACTACGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115837-0022-226-C07C-AH56
CTCTTCGACAAACATGGCGATTCCTACCAGCTTCTCCTCTTACAGGCAATCCTCTTCATT
TTCTGTCTTGTCCTAGCGTTTCTTGCCAAATTTTCCTTTTTAAAAAAAAACTTGTTATGT
TTTCTTTCTGGGTTTTTTCGTTATGCAAATTGCATATACTTTGTGTGCTTCTTCTTATTA
GGCCTTTGCTTTTATTGAACGATTTCCGCCTCTGACTTCTATCGATGCTATTATCGTATA
ATTGGGAATGATTGTCATGTTGCAGCTCTTTACTTTCTCACTTTTACTCACCGCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 04-K115833-0022-226-C07C-8409
GenBank Accession LN488055 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115833-0022-226-C07C-8409
TTGCGGTGAGTAAAGTGAGAAAGTAAAGAGCTGCAACATGACAATCATTCCCAATTATAC
GATAATAGCATCGATAGAAGTCAGAGGCGGAAATCGTTCAATAAAAGCAAAGGCCTAATA
AGAAGAAGCACACAAAGTATATGCAATTTGCATAACGAAAAAACCCAGAAAGAAAACATA
ACAAGTTTTTTTTTAAAAAGGAAAATTTGGCAAGAAACGCTAGGACAAGACAGAAAATGA
AGAGGATTGCCTGTAAGAGGAGAAGCTTGGTAGGAATCGCCATGTTTTGTCTGAAGAGCA
ATTAAGCAAGCAGTTGGGGTTTATTTAATCTTGGCGTAGTAGTTAGTTGCAGAGA

Last Updated on 06.12.2018 16:11