Sequences from confirmation amplicon


Confirmation sequences for line 226F04 and hit At4g10540 are shown.
Line ID 226F04
Locus At4g10540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6515020
Primers used for PCR CR08 - 8474, show primer details
   
Sequence Name 26-K017192-022-226-F04C-CR08
GenBank Accession LN497699 [GenBank]
Sequencing primer gene specific primer, CR08: 5'-TCAGTTTCTGGGAAGTAAGGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K017192-022-226-F04C-CR08
CGATAGCCGATAGACAAGGACAAAACATCAACACCATCATGCATAGCTTCATCCATAGCT
TTCAAGATATCAGCAGATGAACATGTATTTATGTCAAAACGATCTAGATACCAACAAGCT
TTGTACATCGCTATACGAGCACGTGGTGCCCCACCTCTCACAGTCCCTCCAGCTAACCCC
TTGTAGCTTATGCTAGGCACGTAGGAACCACCCGCTATGGTGGCTACATGCGTGCCATGT
CCGCTACGGTCTCTAGGGGAAATAAAGTCAAGTGATTCTGTGGAGTTGAAGCTTTCGTGC
GTCGCAAGAAACCCATTGATGAAGTATTTGGCTCCTATAAGCTTTTTGTTGCACTGAGAG
GAAGTGAAATTCTCTCCTGATACACAGCCTCCTTTCCAGTGGCTTGGCACTGGTCCAATC
CCATTGTCATTAAAGACTTCAGATTCTGGCCAAACTCCTACAACCTCAGAAAATGATTTA
TTGATAGAGGAAACAAAAGCAACAAGAGAACTAAAATCAATCTACAATGTAGCAAAAAAG
AAATGCACAAATAAACACTCAAACCTGAGTCTACAATGCCAATGATAACTTCTTCACCCA
TATTAGTGTCATTTAGAAGATTTTTAGGATTGGCGACGGAAAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTC
   
Sequence Name 30-K017192-022-226-F04C-8409
GenBank Accession LN499513 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K017192-022-226-F04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTTCCGTCGCCAAT
CCTAAAAATCTTCTAAATGACACTAATATGGGTGAAGAAGTTATCATTGGCATTGTAGAC
TCAGGTTTGAGTGTTTATTTGTGCATTTCTTTTTTGCTACATTGTAGATTGATTTTAGTT
CTCTTGTTGCTTTTGTTTCCTCTATCAATAAATCATTTTCTGAGGTTGTAGGAGTTTGGC
CAGAATCTGAAGTCTTTAATGACAATGGGATTGGACCAGTGCCAAGCCACTGGAAAGGAG
GCTGTGTATCAGGAGAGAATTTCACTTCCTCTCAGTGCAACAAAAAGCTTATAGGAGCCA
AATACTTCATCAATGGGTTTCTTGCGACGCACGAAAGCTTCAACTCCACAGAATCACTTG
ACTTTATTTCCCCTAGAGACCGTAGCGGACATGGCACGCATGTAGCCACCATAGCGGGTG
GTTCCTACGTGCCTAGCATAAGCTACAAGGGGTTACCTGGAGGGACTGTGAGAGGTGGGG
CACCACGTGCTCGTATAGCGATGTACAAAGCTTGTTGGTATCTAGATCGTTTTGACATAA
ATACATGTTCATCTGCTGATATCTTGAAAGCTATGGATGAAGCTATGCATGATGGTGTTG
ATGTTTTGTCCTTGTCTATCGGCTATCGTTTTCCTTAC

Last Updated on 06.12.2018 16:11