Sequences from confirmation amplicon


Confirmation sequences for line 227A09 and hit At4g13280 are shown.
Line ID 227A09
Locus At4g13280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7736089
Primers used for PCR 07mb - 8474, show primer details
   
Sequence Name 56-K114067-0022-227-A09C-07mb
GenBank Accession LN509172 [GenBank]
Sequencing primer gene specific primer, 07mb: 5'-TTGACTATATATGTATAAACCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114067-0022-227-A09C-07mb
AGCCGGAAGTTATGGAGCTCCTATCTTCCCAGGGTGACGATGAGACATCGAAGAGGAAAG
TGCTTTTGATTCAATTGCTTCTTAGTCTTGGTCTCGCATTTCATTTTGAAAATGAGATCA
AAAATATACTAGAACATGCTTTTCGAAAGATAGATGATATAACTGGTGATGAAAAGGACT
TGTCTACAATCTCCATCATGTTCCGTGTCTTTAGAACATACGGACACAATTTACCATCAA
GTAAGACAATTGTTTAGTCGGTGGCAACAAATCTAAGAATGTTTTTATCATTTTATTTTG
TAACAGATTACCTGCAATACCATCACAATTAGCAATAGATTTCCACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGAC
ACGATTATTA
   
Sequence Name 31-K114066-0022-227-A09C-8409
GenBank Accession LN500251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114066-0022-227-A09C-8409
GCCTTTACATTGAATATATCCTGTGGAAATCTATTGCTAATTGTGATGGTATTGCAGGTA
ATCTGTTACAAAATAAAATGATAAAAACATTCTTAGATTTGTTGCCACCGACTAAACAAT
TGTCTTACTTGATGGTAAATTGTGTCCGTATGTTCTAAAGACACGGAACATGATGGAGAT
TGTAGACAAGTCCTTTTCATCACCAGTTATATCATCTATCTTTCGAAAAGCATGTTCTAG
TATATTTTTGATCTCATTTTCAAAATGAAATGCGAGACCAAGACTAAGAAGCAATTGAAT
CAAAAGCACTTTCCTCTTCGATGTCTCATCGTCACCCTGGGAAGATAGGAGCTCCATAAC
TTCCGGCTTGAGTATGTCAATCTCTCTTGTAATAACATCTAGTTCCTATTAGGTTTATAC
ATATATAGTCA

Last Updated on 06.12.2018 16:11