Sequences from confirmation amplicon


Confirmation sequences for line 227B02 and hit At5g59430 are shown.
Line ID 227B02
Locus At5g59430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23968852
Primers used for PCR 37ra - 8474, show primer details
   
Sequence Name 85-K115895-0022-227-B02C-37ra
GenBank Accession LN514581 [GenBank]
Sequencing primer gene specific primer, 37ra: 5'-ACGGTAATCATCAGTAACGGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K115895-0022-227-B02C-37ra
ACTCCTCTGCTGGTGCAAGTAATAATTTCTCCACGGTTTCGTATCTGCAACACCAAATAC
ATGTCATTAAAACAAAACCCATCAAATGTATTTAAGATATGAAACAGATAAGAACCCCTT
AGGCAGGCTACTACCTGAATGCCTTGAACCTCCTTTCCAATGTCTAGAAGCCAATATCTT
CCTGATTCTTCTGTCCCCAATACGAGGTACAGTCCTAGGTGAATGTTTTGTAACACGCGG
GTGAATGTACTTGGAAAAGTTTTCATCATCATCTCTACTAAACAAATTTACATCATCCCG
AACGCCATGGGAAACACAGTTCCCAATTGAATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 55-K115894-0022-227-B02C-8409
GenBank Accession LN508906 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K115894-0022-227-B02C-8409
GCATTTCATTGAATTATTCAATTGGGAACTGTGTTTCCCATGGCGTTCGGGATGATGTAA
ATTTGTTTAGTAGAGATGATGATGAAAACTTTTCCAAGTACATTCACCCGCGTGTTACAA
AACATTCACCTAGGACTGTACCTCGTATTGGGGACAGAAGAATCAGGAAGATATTGGCTT
CTAGACATTGGAAAGGAGGTTCAAGGCATTCAGGTAGTAGCCTGCCTAAGGGGTTCTTAT
CTGTTTCATATCTTAAATACATTTGATGGGTTTTGTTTTAATGACATGTATTTGGTGTTG
CAGATACGAAACCGTGGAGAAATTATTACTTGCACCAGCAGAGGAGCTATCCTATCAAGA
AAAGGAAAAACTTTGACCACATATCTGATTCCGTTACTGATGATTACCG

Last Updated on 06.12.2018 16:11