Sequences from confirmation amplicon


Confirmation sequences for line 227D02 and hit At1g74030 are shown.
Line ID 227D02
Locus At1g74030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27841154
Primers used for PCR MH22 - 8474, show primer details
   
Sequence Name 30-K020068-022-227-D02C-8409
GenBank Accession LN499535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K020068-022-227-D02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTTTTTCTCATACTTATTTTCTT
TAAGGAACCAAGCTGATGTCGATGCTCTTATGCTGGAACTGGATGGGACCCCAAACAAGT
CGAAACTCGGGGCTAATGCGATATTAGGAGTGTCATTGAGCGTTTGCAGGGCAGGTGCTG
GAGCTAAAGGAGTGCCTTTGTACAAACACATCCAGGAAACATCAGGAACAAAGGAGCTTG
TCATGCCAGTTCCTGCATTCAATGTGATCAATGGAGGCAGTCATGCTGGGAATAGTTTGG
CTATGCAAGAGTTTATGATACTACCTGTAGGAGCTACCTCATTCTCGGAGGCCTTCCAGA
TGGGAAGTGAAGGTCAGTTAGTGATAACTGGGTTTAACTTGCTAGAACTTAAGAAACTAT
GGCGGCTCCTAATTTTTCTTGAGTTTGCAATGAAATGTC
   
Sequence Name 29-K020068-022-227-D02C-MH22
GenBank Accession LN499097 [GenBank]
Sequencing primer gene specific primer, MH22: 5'-TCTGTTTTTCTGACATTTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K020068-022-227-D02C-MH22
ACTCAAGTAAAAATTAGGAGCCGCCATAGTTTCTTAAGTTCTAGCAAGTTAAACCCAGTT
ATCACTAACTGACCTTCACTTCCCATCTGGAAGGCCTCCGAGAATGAGGTAGCTCCTACA
GGTAGTATCATAAACTCTTGCATAGCCAAACTATTCCCAGCATGACTGCCTCCATTGATC
ACATTGAATGCAGGAACTGGCATGACAAGCTCCTTTGTTCCTGATGTTTCCTGGATGTGT
TTGTACAAAGGCACTCCTTTAGCTCCAGCACCTGCCCTGCAAACGCTCAATGACACTCCT
AATATCGCATTAGCCCCGAGTTTCGACTTGTTTGGGGTCCCATCCAGTTCCAGCATAAGA
GCATCGACATCAGCTTGGTTCCTTAAAGAAAATAAGTATGAGAAAAAATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11