Sequences from confirmation amplicon


Confirmation sequences for line 227G01 and hit At3g20770 are shown.
Line ID 227G01
Locus At3g20770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7262138
Primers used for PCR MM53 - 8474, show primer details
   
Sequence Name 29-K020131-022-227-G01C-MM53
GenBank Accession LN499098 [GenBank]
Sequencing primer gene specific primer, MM53: 5'-TCTCTGTTTGGCTTTCTCTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K020131-022-227-G01C-MM53
ATGAATTCGAGTTTCTGCTGGGACTTCTTCTTTGACAGGAAAGTCATGCATTTTAGCAAC
CATCCCAAAGTTTGAAGAATTCATAACTTTTTCTGGCTTGAGCTCTTCCACTTCATAGTG
AGACTCCTTCTCGAAACCTTCAACATCGTATTGACTGCAATCATTCATCAGAAGCGAGCA
ACTTCCACCAGACAGAGAAAGAGGTGGACATGACTCGGGATAAAGCTCTCTAGCCAAGGA
CTCTTCTTGGTTAATAATAGCAAGCCAGGTAGCACTCTCTTTAGCAGTCATCTTATCCTG
CAAACATTTAGATTGCCTCACGAGCTTACGGATCTTAGCAATATCAGGAAACATATGCTT
GATAACCGCAGTCAAAACGCCGACTTTCCACGCCTTCTTCAAATCATGAGGCTTCTTGTA
AGGTGCAGGACCTTGATCTTTAGGCAAACCAAGTTGAGGCCACCAATCCTCTTTCCCATT
AGGCCACCACGGAGGAGGAACTCCTTTCTCCAAAGGAAAACGTCTCTGAGGAGGATCACA
GTGTTGCATCAACGCAGACAAAAGCGATCCAAGAGTCGTGTCTTGAAGCTCTTGCAAGGT
ATGAGGAGTCGGTCCAATCGGGTTATTACCTTCATGAATCCCCGGGATATTATTCTCCGC
TTGATACTTGGTAATAGCTAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
   
Sequence Name 30-K020131-022-227-G01C-8409
GenBank Accession LN499536 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K020131-022-227-G01C-8409
TAGATTTCCCGGACATGTAGATTTAGCTATTACCAAGTATCAAGCGGAGAATAATATCCC
GGGGATTCATGAAGGTAATAACCCGATTGGACCGACTCCTCATACCTTGCAAGAGCTTCA
AGACACGACTCTTGGATCGCTTTTGTCTGCGTTGATGCAACACTGTGATCCTCCTCAGAG
ACGTTTTCCTTTGGAGAAAGGAGTTCCTCCTCCGTGGTGGCCTAATGGGAAAGAGGATTG
GTGGCCTCAACTTGGTTTGCCTAAAGATCAAGGTCCTGCACCTTACAAGAAGCCTCATGA
TTTGAAGAAGGCGTGGAAAGTCGGCGTTTTGACTGCGGTTATCAAGCATATGTTTCCTGA
TATTGCTAAGATCCGTAAGCTCGTGAGGCAATCTAAATGTTTGCAGGATAAGATGACTGC
TAAAGAGAGTGCTACCTGGCTTGCTATTATTAACCAAGAAGAGTCCTTGGCTAGAGAGCT
TTATCCCGAGTCATGTCCACCTCTTTCTCTGTCTGGTGGAAGTTGCTCGCTTCTGATGAA
TGATTGCAGTCAATACGATGTTGAAGGTTTCGAGAAGGAGTCTCACTATGAAGTGGAAGA
GCTCAAGCCAGAAAAAGTTATGAATTCTTCAAACTTTGGGATGGTTGCTAAAATGCATGA
CTTTCCTGTCAAAGAAGAAGTCCCAGCAGGAAACTCGGAATTCATGAGAAA

Last Updated on 06.12.2018 16:11